HIF-1α和c-met在喉鳞癌中的表达及其与微血管密度的关系.docVIP

HIF-1α和c-met在喉鳞癌中的表达 及其与微血管密度的关系 孙旭 娄卫华 郑州大学第一附属医院 郑州 450052 摘 要 目的：探讨观察缺氧诱导因子-1α(hypoxia-inducible factor l alpha, HIF-1α)、肝细胞生长因子受体(hepatocyte growth factor receptor, c-met)在喉鳞状细胞癌发生发展和淋巴转移中的作用。方法：采用免疫组化SP法，检测HIF-1α和c-met在喉鳞状细胞癌不同分化类型中的表达(58例)，并测定微血管密度(microvessel density ,MVD)，观察HIF-1α、c-met和MVD之间的联系。结果：HIF-1α、c-met在喉癌中阳性区平均积分光密度(the average integrated optical density, AIOD)及MVD随分化程度降低而增高(P0.05)。颈淋巴结转移组AIOD及MVD明显高于未转移组(P0.05)。HIF-1α与c-met在喉癌中的表达呈明显正相关(P0.001)。HIF-1α、c-met与MVD也呈明显正相关(P0.001)。结论：HIF-1α、c-met在喉鳞状细胞癌的发生、发展、淋巴结转移中起重要作用，且两者存在有协同作用。HIF-1α、c-met可能作为治疗喉鳞癌的潜在靶点。 关键词：喉鳞癌;缺氧诱导因子-1α;肝细胞生长因子受体;微血管密度 中图分类号：R767 The Significance and Relationship between HIF-1α,C-met and Microvessel Density Expression in Laryngeal Carcinoma Xu Sun Weihua Lou The First Affiliated Hospital of Zhengzhou University Zhengzhou 450052 ABSTRACT Objective：To evaluate the role of HIF-1α and C-met in tumorigenesis and matastasis of laryngeal squamous cell carcinoma. Methods: Immunohistochemical technique (streptavidin peroxidase method) was applied to examining expression of HIF-1α,C-met and CD34 in three types of differentiation of laryngeal squamous cell carcinoma(58 tissue samples). Investigate the relationship between HIF-1α,C-met and CD34.Results: The average integrated optical density( AIOD) of positive area and MVD in poorly differentiated laryngeal carcinoma were significantly higher than that in well differentiated laryngeal carcinoma and moderately differentiated laryngeal carcinoma(P0.05), The AIOD of positive area and MVD in moderately differentiated laryngeal carcinoma were significantly higher than that in well differentiated laryngeal carcinoma(P0.05). The AIOD of positive area and MVD in group with lymphnode metastasis were significantly higher than that in group without lymphnode metastasis.The expression of HIF-1α and c-met were positively correlated (P0.001 ).The expression of HIF-1α and c-met positively correlated with MVD expression (P0.001).Conclusion: The expressi