sex, subdivision, and domestic dispersal of trypanosoma cruzi lineage i in southern ecuador性别细分,国内传播鲁兹锥体血统我在厄瓜多尔南部.pdfVIP

Sex, Subdivision, and Domestic Dispersal of Trypanosoma cruzi Lineage I in Southern Ecuador ´ ˜ 1 2 1 2 1,3 Sofıa Ocana-Mayorga , Martin S. Llewellyn , Jaime A. Costales , Michael A. Miles , Mario J. Grijalva * ´ ´ 1 Centro de Investigacion en Enfermedades Infecciosas, Pontificia Universidad Catolica del Ecuador, Quito, Ecuador, 2 London School of Hygiene and Tropical Medicine, London, United Kindom, 3 Biomedical Sciences Department, College of Osteopathic Medicine, Tropical Disease Institute, Ohio University, Athens, Ohio, United States of America Abstract Background: Molecular epidemiology at the community level has an important guiding role in zoonotic disease control programmes where genetic markers are suitably variable to unravel the dynamics of local transmission. We evaluated the molecular diversity of Trypanosoma cruzi, the etiological agent of Chagas disease, in southern Ecuador (Loja Province). This kinetoplastid parasite has traditionally been a paradigm for clonal population structure in pathogenic organisms. However, the presence of naturally occurring hybrids, mitochondrial introgression, and evidence of genetic exchange in the laboratory question this dogma. Methodology/Principal Findings: Eighty-one parasite isolates from domiciliary, peridomiciliary, and sylvatic triatomines and mammals were genotyped across 10 variable microsatellite loci. Two discrete parasite populations were defined: one predominantly composed of isolates from domestic and peridomestic foci, and another predominantly c