大鼠基质金属蛋白酶2试剂盒说明书（Rat matrix metalloproteinase 2 Kit）.docVIP

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大鼠基质金属蛋白酶2试剂盒说明书（Rat matrix metalloproteinase 2 Kit）.doc

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大鼠基质金属蛋白酶2试剂盒说明书（Rat matrix metalloproteinase 2 Kit）

大鼠基质金属蛋白酶2试剂盒说明书（Rat matrix metalloproteinase 2 Kit） Matrix metalloproteinase -2 (MMP-2) enzyme-linked immunosorbent assay (ELISA) in rats Kit instructions This reagent is for research purpose only: this kit is used for the determination of rat serum and plasma, Content of matrix metalloproteinase -2 (MMP-2) in supernatant and related liquid samples. Experimental principle: The expression of matrix metalloproteinase 2 (MMP-2) in rats was measured by double antibody sandwich method. The use of pure Rat matrix metalloproteinase 2 (MMP-2) antibodies were coated with microporous plates to make solid phase antibodies to the microporous envelope of the mAb Sub - matrix metalloproteinase 2 (MMP-2) was added and then conjugated with HRP labeled MMP-2 antibody to form antibody Enzyme labeled antibody complexes were washed with substrate TMB to give color. TMB was converted into HRP under the catalysis of the enzyme And under the action of acid into the final yellow. Color depth and matrix metalloproteinase 2 (MMP) in the sample Positive correlation. Measured absorbance in the 450nm wavelength with ELISA (OD), calculate the sample through the standard curve Matrix metalloproteinase 2 (MMP-2) concentration. Kit composition: The kit is composed of 48 holes and is equipped with 96 holes 1 copies of the instruction manual, 1 copies The sealing plate is 2 (48) and 2 (96) Sealed bags 1, 1 The enzyme labeled plates are 1 x 481 x 962-8 degrees Standard: 450 g/L0.5ml * 1 bottles, 0.5ml * 1 bottles, 2-8 degrees guarantee Standard diluent 1.5ml * 1 bottles, 1.5ml * 1 bottles, 2-8 DEG C protection Enzyme labeled reagent 3ml * 1 bottles, 6ml * 1 bottles, 2-8 DEG C protection Sample dilution 3ml * 1 bottles, 6ml * 1 bottles, 2-8 DEG C protection Chromogenic agent A liquid, 3ml * 1 bottles, 6ml * 1 bottles, 2-8 degrees guarantee Chromogenic agent B liquid, 3ml * 1 bottles, 6ml * 1 bottles, 2-8 degrees guarantee Termination Liquid 3ml * 1 bottles, 6ml * 1 bottles, 2-8 degrees guaranteed Conc