植物病原细菌检测细菌病害诊断方法.pdfVIP

7 3-4 V l17 N 13-4 2009 11 J urnal f Fungal Research N v. 2009 * 1 2 刘学敏 , 孟玉芹 ( 1. 东北农业大学农学院, 哈尔滨 150030; 2. 黑河出入境检验检疫局, 黑河164300) : 植物病害诊断是通过病菌鉴定和致病性测定等步骤实现的在过去的10 年 用于细菌鉴定的基因 组技术的快速发展极大地简化和促进了病菌的检测和鉴定, 但是DNA 分子检测方法不能完全取代传统的培 养检验和表型检验方法文中介绍了未显示病害症状的植物或植物产品中已知细菌的免疫检测和基因组 DNA 检测方法, 以及细菌病害诊断鉴定的新方法 : 病原细菌; 免疫检测; DNA 分子检测; 基因组探针; 多相分析诊断 : S43211 : A : 1672-3538( 2009) 03-04-0211-07 Approaches of Plant Pathogenic Bacteria Detection and Bacterial Disease Diagnosis 1 2 LIU Xue-min , MENG Y u-qin ( 1. College fo Agronomy, NortheastAgricultural University, Harbin 150030, China; 2. Heihe Entry-Ex- it Ins ection and Quarantine Bureau, Heihe 164300, China) Abstract: Plant disease diagn sis is based n a number f fact rs, including lab rat ry tests f r path gen identificati n and path genicity tests. Rapid devel pment f gen mic techniques f r characterizati n f bacteria ver the past decade has greatly simplified and impr ved path gen detecti n and identificati n, but DNA-based meth ds have n t yet entirely replaced traditi nal culture and phen typic tests. The paper summarized rapid immun diagn stic and DNA-based detecti n meth ds f r kn wn bacterial path gens in plants r plant pr ducts, which ften manifest n sympt ms f disease, and new meth ds f r identifying and characterizing bacteria disease diagn sis. Key words: path genic bacteria; immun diagn sis; DNA-based detecti n; gen mic pr be; p lyphasic analysis diagn sis 10 , 500 ,