2-(2-苯并呋喃基)-2-咪唑啉对星形胶质细胞糖氧剥夺损伤保护及.docVIP

2-(2-苯并呋喃基)-2-咪唑啉对星形胶质细胞糖氧剥夺损伤保护及 　作者：田继沙，韩钊，张红霞，肖美娟，杨金龙，程朝辉，王小同 【摘要】 目的 ：探讨2-(2-苯并呋喃基)-2-咪唑啉(2-BFI)对星形胶质细胞糖氧剥夺(oxygen-glucosedeprivation，OGD)损伤的保护作用及脂质过氧化的影响。方法：制成星形胶质细胞糖氧剥夺损伤模型，分为正常对照组、OGD组、OGD+2-BFI干预组(浓度分别为0.75、1.5、3、6和12μg/mL)，测定星形胶质细胞存活率，选出抗OGD损伤最佳浓度，以该浓度进一步观察2-BFI对脂质过氧化产物丙二醛(MDA)和还原型谷胱甘肽(GSH)含量的影响。结果：星形胶质细胞在OGD损伤后，不同浓度的2-BFI均能提高细胞存活率，其中1.5μg/mL的效果最佳;予1.5μg/mL 2-BFI干预OGD组能显著降低MDA含量，增加GSH含量。结论：2-BFI能保护星形胶质细胞OGD损伤，且有抑制脂质过氧化的作用。 【关键词】 咪唑啉受体;2-BFI;糖氧剥夺;脂质过氧化;大鼠 　Abstract: Objective: To explore the neuroprotective effects and lipid peroxidation of 2-(2-benzonfuranyl)-2-imidazoline on oxygen-glucose deprivation of cultured rat corticalastrocytes. Methods: The model of oxygen-glucose deprivation of cultured rat cortical astrocyteswas established.They were designed into three groups: normal control group，oxygen-glucosedeprivation(OGD) group and oxygen-glucose deprivation+2-BFI intervention group (the　　concentration of drug was 0.75，1.5，3，6 and 12μg/mL)，to determine the cell survival rate　　of astrocytes，the best drug concentration was chosen，then the best drug density was used to　measure the content of malondialdehyde(MDA) and reduced glutathione (GSH). Results: When　astrocytes were deprived of oxygen and glucose，different concentration of 2-BFI couldsignificantly raised the cell survival rate，and the effect of 1.5μg/mL was the best;1.5μ　　g/mL 2-BFI intervention group could significantly reduced MDA level，and raised the content of　　reduced glutathione. Conclusion: 2-BFI can effectively protect astrocyte injury afteroxygen-glucose deprivation，and inhibit lipid peroxidation. 　　Key words: imidazoline receptors;2-BFI;oxygen-glucose deprivation;lipid peroxidation;rats 　　1984年Bousquet首次提出咪唑啉受体(imida-zoline receptors，IR)概念，迄今的研究认为该受体有两种亚型，一种是与可乐定有高亲和力的咪唑啉I1受体(I1R)，主要存在于延髓头端腹外侧，与血压的调节有关;另一种是与咪唑克生有高亲和力的咪唑啉I2受体(I2R)，在人体广泛存在于神经系统、心血管系统、肾脏等组织。2-(2-苯并呋喃基)-2-咪唑啉〔2-(2-benzofu-ranyl)-2-imidazoline， 2-BFI〕是近年来才新发现的高亲和力I2R配体。我们的前期研究发现2-BFI能够缩减大鼠脑缺血再灌注损伤后的梗死体积，降低神经功能损