MCP1基因A2518G多态性及不稳定性心绞痛关系初步分析.docVIP

MCP1基因A2518G多态性及不稳定性心绞痛关系初步分析 　 作者：师干伟，何国平，戚传平，高磊，戚猛，沈丹丹，钱志宏，许联红 【摘要】 目的： 探讨中国苏南地区汉族人群单核细胞趋化蛋白1（MCP1）基因启动子A2518G单核苷酸多态性与不稳定性心绞痛（UAP）的相关性。方法： 采用病例对照研究方法，选择临床确诊的UAP患者172例为病例组和经冠脉造影检查证实无冠脉病变者156例为对照组。利用聚合酶链式反应限制性酶切片段长度多态性技术（PCRRFLP）对MCP1基因 A2518G行多态性检测，并统计各基因型及等位基因的频率分布。结果： 与对照组相比，UAP组的AA，AG和GG基因型频率差异均无统计学意义（P值分别为0.067，0.825和0.281）。G等位基因频率在UAP组和对照组分别为54.36%和61.22%，两组间差异无统计学意义（P=0.069）。对多个相关因素行多元Logistic回归分析显示，MCP1基因A2518G单核苷酸多态性与UAP的发病无相关性（Pgt;0.05）。结论： 在中国苏南地区汉族人群中，MCP1基因A2518G 单核苷酸多态性与UAP无显著相关性。 【关键词】 不稳定性心绞痛； 单核细胞趋化蛋白1； 单核苷酸多态性 　　［Abstract］Objective: To investigate the possible association between the monocyte chemoattractant protein1(MCP1) gene A2518G single nucleotide polymorphism(SNPs) in the promoter region with unstable angina pectoris(UAP) in Chinese Han population of Sunan region. Methods： This study was conducted with a casecontrol design including 172 patients with UAP(UAP group) and 156 control subjects who were free from coronary artery disease by Coronary Angiography checks(control group). Polymerase chain reactionrestriction fragment length polymorphism(PCRRFLP) was used for the detection of the A2518G polymorphism in MCP1 gene，and then the frequency of genetype was statistically analyzed. Results： Compared with the control group, the frequencies of AA，AG and GG genotype were not significantly different in UAP group(P was 0.067，0.825 and 0.281, respectively). The prevalence of G allele was 54.36% and 61.22% respectively in the UAP group and control group; there was no difference between them in statistics(P=0.069). Multivariate logistic regression analysis indicated that there was no significant correlation between MCP1 gene A2518G polymorphism with UAP(Pgt;0.05). Conclusion： Our data shows that MCP1 gene A2518G polymorphism is not associated with the risk of UAP in the Chinese Han population of Suwan region. 　　［Key words］unstable angina pectoris; monocyte chemoattractant protein1; single nucleotide polymorphism