中国恒河猴（Macaca mulatta）外周血Th17细胞及其在SHIV感染后.docVIP

中国恒河猴（Macaca mulatta）外周血Th17细胞及其在SHIV感染后 　【摘要】 　　目的: 对正常中国恒河猴(Macaca mulatta)及嵌合猿猴/人免疫缺陷病毒（SHIV）感染过的中国恒河猴外周血中CD4+IL17A+和CD4-IL17A+T淋巴细胞的分布频率进行初步观察。方法: 用荧光染料标记的单克隆抗体（mAb）对10只中国恒河猴的外周血或PMA+Ionomycin刺激后的PBMC细胞表面的CD3、 CD4、 CD8及细胞内IL17A进行免疫荧光染色。用FACScalibaur获取染色后的样品, 所得数据用CellQuest进行分析。结果: 在6只正常中国恒河猴个体中均能检测到IL17A+淋巴细胞, 未经刺激培养的样品中IL17A+细胞频率很低, 但在短期刺激培养后淋巴细胞中具有约1.4%的淋巴细胞为IL17A+细胞, 明显多于刺激前的IL17A+淋巴细胞; 在短期刺激培养后CD3+CD4+淋巴细胞中大约有2.7%的IL17A+细胞或CD4+Th17细胞。对4只SHIV感染个体中Th17细胞的初步分析未发现它们与正常个体间的统计学差异。结论: 与人类相似, 中国恒河猴的外周血中也存在一定比例的Th17细胞, 为进一步利用恒河猴AIDS动物模型分析HIV/AIDS与Th17细胞的关系提供了基础。 【关键词】 中国恒河猴 外周血单个核细胞 IL 17A+细胞 CD4+Th17 猿猴/人免疫缺陷病毒感染 　　[Abstract] AIM: To observe CD4+IL17+cells in the peripheral blood of Chinese rhesus macaques. METHODS: Peripheral blood samples were collected from normal and SHIV infected rhesus macaques. Surface CD3, CD4, CD8 and intracellular IL17A expression of peripheral blood (with or without PMA+Ionomycin stimulation) were detected by immunostaining with fluorochrome labeled monoclonal antibodies. The samples were acquired with FACScalibur and the data were analyzed with CellQuest. RESULTS: IL17A+cells were observed in all the 6 normal rhesus macaques but there were very few before cultured with PMA+Ionomycin. About 1.4% of lymphocytes were IL17A+cells after stimulation and shortterm cultivation, and about 2.7% of CD3+CD4+ lymphocytes were IL17A+cells, i.e. CD4+ Th17 cells. Th17 cells in the 4 SHIV infected rhesus monkeys were also examined. The number of the Th17 cells detected after stimulation and shortterm cultivation was not significantly different from that of normal monkeys. CONCLUS ION: Similar to human race, Chinese rhesus macaques possess a subset of CD4+IL17A+cells, suggesting these rhesus monkey can be used to study the relationship between Th17 cells and HIV/AIDS. 　　[Keywords]Chinese rhesus macaque; PBMC; IL17A+ cell; CD4+Th17 cell; SHIV infection 　　在对鼠类实验动物的研究中发现, 鼠类动物未接触抗原的CD4+T淋巴细胞在抗原激活后能够分化产生不同表型和功能的辅助性T淋巴细胞。CD4+辅助性T