千里光水浸液对大肠埃希菌R质粒消除作用.docVIP

千里光水浸液对大肠埃希菌R质粒消除作用 　 作者：张文平 曹镐禄 张文书 黄真 【摘要】 　　目的探讨千里光对大肠埃希菌R质粒消除作用。方法对尿路感染患者尿液中分离的大肠埃希菌R质粒进行检测，并选用中药千里光水浸液对该质粒进行了体外、体内消除实验。体外实验：千里光水浸液亚抑菌浓度分别作用大肠埃希菌24，48，72 h后，影印培养，计数消除子数；体内实验：建立肠道去污染小鼠动物模型后，经口感染大肠埃希菌，同时给千里光水浸液，灌胃给药，每只2 mg/d，分别于给药后24 ，48，72 h后处死小鼠，分离培养细菌，计数消除子数。消除子经质粒抽提琼脂糖凝胶电泳鉴定。结果体外药物作用24，48，72 h后，R质较消除率分别为：0.2%，2.2%，3.8 %；SDS对照组分别为12.1%，14.9%，23.7%，体内药物作用24，48，72 h R质粒消除率分别为0%，3.1%，14.4%，对照组分别为0%，0.1 %，0.1%。结论中药千里光水浸液对大肠埃希菌R质粒于体内、体外均具有消除作用，体内消除作用强于体外。 【关键词】 千里光 大肠埃希菌 R质粒 消除 　　Abstract:ObjectiveTo explore the elimination effect of Senecio scandens Buch-Ham (SSB) Liquid Extracts on R plasmid (RP) of Escherichia coil．MethodsRP of E.coil were isolated from the urine of urinary tract infection patients, and the elimination test of SSB extracts on these plasmids was performed in vitro and in vivo. After sub-bacteriostatic concentration of SSB extracts treated E.coil in vitro for 24 h, 48h and 72h. Replica plating and counting of colonies resisting to antibiotics were carried out. Germ-free mice in intestine were orally inoculated with E.coil, then water decoct agent of SSB was given orally in 2 mg/d per mouse, Eliminant of E.coil was identified by replica plating and colony counting after 24 h, 48h and 72 h, respectively．RP eliminated bodies were identified through electrophoresis with agarose gel. ResultsAfter drug acted on E.coil 24 h, 48h and 72h in vitro respectively, elimination rate of RP in SSB tested group were 0.2%, 2.2%, 3.8%, while in control SDS group were 12.1%, 14.9%, 23.7%，respectively; 24 h, 48h, 72h in vivo, elimination rate of RP in test group were 0, 3.1%, 14.4%, and in control group were 0, 0.1%, 0.1%, respectively.　ConclusionSSB has strong function to eliminate RP of E.coil in vivo and in vitro. The eliminative effect is stronger in vivo than in vitro. 　　Key words:Senecio scandens Buch-Ham; Escherichia coil; R plasmids; Elimination 　　大肠埃希菌作为条件致病菌是医院内感染最常见病原菌之一，其耐药菌株引起的感染日益增多。细菌的耐药性由染色体或耐药质粒(R质粒)介导，耐药质粒可通过接合、转化、转导等方式在不同菌种之间传递，使敏感菌成为耐药株，造成了R质粒的流行