双波长比值光谱法测定复方益康宁片二组分含量.docVIP

双波长比值光谱法测定复方益康宁片二组分含量 　 作者：黄喜茹，刘伟娜，周亚君，曹冬 【关键词】 双波长比值光谱法 Dualwavelength ratio spectrophotometry for determination of procaine hydrochloride and vitamin B6 in compound Yikangning tablets 　　【Abstract】 AIM: To establish dual-wavelength ratio spectrophotometry for determination of procaine hydrochloride and vitamin B6 in compound Yikangning tablets. METHODS: The ratio spectra was determined and the wavelength pair were selected at the peak point or valley point of ratio spectra , to eliminate the interference of other components. RESULTS: Linearity between the absorbance and concentration of procaine hydrochloride and vitamin B6 was obtained in the range of 5.0-50.0 mg/L under the optimum conditions . The average recoveries and relative standard deviations(RSD) of procaine hydrochloride and vitamin B6 were 99.5%, 1.2% and 98.3%, 0.8%, respectively. CONCLUSION: The method was simple , rapid and reproducible , the interference of two components on each other may be eliminated and the results were satisfactory. 　　【Keywords】 dualwavelength ratio spectrophotometry; procaine hydrochloride; vitamin B6; Yikangning tablet 　　【摘要】 目的：建立复方益康宁片中盐酸普鲁卡因和维生素B6的双波长比值光谱测定法. 方法：绘制盐酸普鲁卡因和维生素B6的比值光谱，选择其峰点或谷点作为测定波长对有效地消除共存组分的干扰，以实现对被测组分的准确测定. 结果：在选定的实验条件下，盐酸普鲁卡因和维生素B6在5.0～50.0 mg/L范围线性关系良好，平均回收率分别为99.5%, 98.3%和相对标准偏差(RSD)分别为1.2%, 0.8%. 结论：该方法简便、快速、重现性好，结果满意. 　　【关键词】 双波长比值光谱法; 盐酸普鲁卡因；维生素B6; 复方益康宁 　　0引言 　　复方益康宁片是用于神经系统平衡与调节的药物，临床用于治疗更年期综合征、神经衰弱和植物神经功能失调等症. 它的主要成分是盐酸普鲁卡因和维生素B6. 山东省药品标准［1］采用比色法测定二组分的含量，操作繁琐费时. 另报道有二阶导数分光光度法［2］和联立方程组新解法［3］,我们采用双波长比值光谱法［4］同时测定二组分的含量，可有效消除共存组分的干扰，操作简便快捷，结果准确，重现性好. 　　1材料和方法 　　1.1材料 　　岛津UV2201型全自动紫外分光光度计; 十万分之一电子天平. 盐酸普鲁卡因、维生素B6对照品（河北省药检所提供，105℃干燥至恒重），溶剂为0.1 mol/L盐酸(下称溶剂), 其他试剂均为分析纯，复方益康宁片（本院药剂室制备）. 　　1.2方法 　　1.2.1基本原理在含有a和b的复方药物中，如二组分在整个波长范围服从朗伯比耳定律，则混合组分的吸收光谱可用方程表示为：Aλi=KaλiCa+KbλiCb①Aλi为混合物在λi处的吸光度；Kaλi，Kbλi为a，b在波长λi处的吸收系数；Ca，Cb为混合物中a和b的浓度. 若b为干扰组分，在对应波长处用标准浓度为Cb′的干扰组分吸收光谱去除①式：Aλi〖〗KbλiCb′=Kaλi〖〗Kbλi#12539;Ca〖〗Cb′+Cb〖〗Cb′② ②式为混合物相对于浓度为Cb′的b组分的比值光谱，可看出在混合物中，b组分（任意