原创力文档a comparative study of protein synthesis in in vitro systems from the prokaryotic reconstituted to the eukaryotic extract-based蛋白质合成的比较研究在体外系统从原核重组真核extract-based.pdfVIP
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a comparative study of protein synthesis in in vitro systems from the prokaryotic reconstituted to the eukaryotic extract-based蛋白质合成的比较研究在体外系统从原核重组真核extract-based
BMC Biotechnology BioMed Central
Research article Open Access
A comparative study of protein synthesis in in vitro systems: from
the prokaryotic reconstituted to the eukaryotic extract-based
Jason R Hillebrecht1,2 and Shaorong Chong*1
Address: 1New England Biolabs, 240 County Road, Ipswich, MA 01938, USA and 2Eedetics, 1200 Soldiers Field Road, Boston, MA 02134, USA
Email: Jason R Hillebrecht - jrhilleb@; Shaorong Chong* - chong@
* Corresponding author
Published: 29 July 2008 Received: 19 March 2008
Accepted: 29 July 2008
BMC Biotechnology 2008, 8:58 doi:10.1186/1472-6750-8-58
This article is available from: /1472-6750/8/58
© 2008 Hillebrecht and Chong; licensee BioMed Central Ltd.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0),
which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Background: Cell-free protein synthesis is not only a rapid and high throughput technology to
obtain proteins from their genes, but also provides an in vitro platform to study protein translation
and folding. A detailed comparison of in vitro protein synthesis in different cell-free systems may
provide insights to their biological differences and guidelines for their applications.
Results: Protein synthesis was investigated in vitro in a reconstituted prokaryotic system, a S30
extract-based system and a eukaryotic system. Compared to the S30 sy
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