a comparison of real-time pcr and reverse line blot hybridization in detecting feline haemoplasmas of domestic cats and an analysis of risk factors associated with haemoplasma infections比较实时pcr和反向线污点杂交检测国内猫和猫haemoplasmas分析haemoplasma感染有关的危险因素.pdfVIP

A comparison of real-time PCR and reverse line blot hybridization in detecting feline haemoplasmas of domestic cats and an analysis of risk factors associated with haemoplasma infections Georges et al. Georges et al. BMC Veterinary Research 2012, 8:103 /1746-6148/8/103 Georges et al. BMC Veterinary Research 2012, 8:103 /1746-6148/8/103 RESEARCH ARTICLE Open Access A comparison of real-time PCR and reverse line blot hybridization in detecting feline haemoplasmas of domestic cats and an analysis of risk factors associated with haemoplasma infections 1* 2 1 1 1 Karla Georges , Chuckwudozi Ezeokoli , Tennille Auguste , Nisshi Seepersad , Akua Pottinger , Olivier Sparagano3 and Séverine Tasker4 Abstract Background: Three species of feline haemoplasma are recognised: Mycoplasma haemofelis (Mhf), ‘Candidatus Mycoplasma haemominutum’ (CMhm) and ‘Candidatus Mycoplasma turicensis (CMt). This study compared a reverse line blot hybridization (RLB) assay for simultaneous detection of Mhf, CMhm with three separate quantitative real- time polymerase chain reaction (qPCR) assays used for diagnosis of Mhf, CMhm and CMt. The RLB and qPCR assays were applied to DNA extracted from blood samples collected from 154 cats from Trinidad and Tobago. Results: CMhm and Mhf DNA were detected using both RLB and qPCR. CMt DNA was detected by qPCR only. Comparing RLB and qPCR for the detection of CMhm DNA, 40 (26.3%) and 48 (31.6%) cats, respectively, were positive. The difference was more marked for Mhf, with RLB detecting a total of only 11 (7.2%) positive cats whereas qPCR detected 41 (27.0%) positive cats. Using q