大鼠肝癌发生过程中bFGF表达及意义.docVIP

大鼠肝癌发生过程中bFGF表达及意义 　 作者;白咸勇，贾秀红，刘同慎，李笑岩 【摘要】 目的 探讨碱性成纤维细胞生长因子(basic fibroblast growth factor，bFGF)在肝癌发生过程中的表达及意义。方法 采用免疫组织化学ABC法对二乙基亚硝胺(DEN)诱发大鼠肝癌发生过程中碱性成纤维细胞生长因子表达情况进行观察。结果 DEN诱发大鼠肝癌发生率为100%。正常大鼠肝细胞bFGF表达为阴性，于诱癌第8周，肝小叶近门管区血管附近的少量肝细胞呈bFGF阳性表达，并可见门管区血管内皮细胞和成纤维细胞也呈bFGF阳性表达，随着诱癌进程的发展，bFGF阳性肝细胞的数量逐渐增多，至诱癌第18周，癌结节内大部分肝癌细胞均呈bFGF阳性表达。结论 bFGF与肝癌的发生和发展具有相关性。 【关键词】 bFGF；大鼠；肝癌发生；免疫组织化学 The expression and significance of basic fibroblast growth factor(bfGF) during hepatocarcinogenesis in the rats 【Abstract】 Objective To investigate the expression and significance of basophilic fibroblast growth factor during hepatocarcinigenesis in the rats.Methods Hepatoma was induced in the rats by administration of diethylnitrosamine (DEN)，the expression of basic fibroblast growth factor (bFGF) was detected with immunohistochemical technique.Results The incidence of hepatocarcinogenesis induced by DEN was 100%，the expression of bFGF was not detected in hepatocytes of normal rats，a few of bFGF positive hepatocytes were detected near the fibrous septums between hepatic lobules at 8th week after DEN treatment，and the positive expression of bFGF was also detected in the endothelial cells of blood vessels and the fibroblast，the number of bFGF positive hepatocytes increased along with the process of carcinogenesis，the majority of hepatocytes of basophilic nodules expressed bFGF at 18th week after DEN administration.Conclusion bFGF is associated closely with the development and progression of hepatic carcinoma. 【Key words】 bFGF;rat;hepatocarcinogenesis;immunohistochemistry 恶性肿瘤的发生、发展、浸润及转移均依赖于肿瘤新生血管的生成［１］。在肿瘤血管生成过程中，促血管生长因子扮演着重要角色，其中碱性成纤维细胞生长因子(basic fibroblast growth factor，bFGF)更为引人关注［２］。肝细胞癌(hepatocellular carcinoma，HCC)是最常见的恶性肿瘤之一，具有高侵袭、易于早期转移、预后差的临床病理特征。但有关bFGF在肝癌发生发展过程中的表达及意义的报道较少。为了解bFGF在肝癌发生发展过程的表达及意义，探讨其与肝癌生物学行为的关系。本实验采用免疫组织化学方法，对二乙基亚硝胺(DEN)诱发大鼠肝癌过程中bFGF表达的变化进行了观察，以便为肝癌发生和防治研究提供实验依据。 1 材料与方法 1.1 肝癌动物模型的建立和病理学检查 雄性Wistar大鼠(由中国科学院上海实验动物中心提供)30只，体重100～１５０g，动物随机分为两组，实验组15只，