受白叶枯病菌诱导的水稻抗病相关基因.pdfVIP

南京农业大学学报 　2003 , 26 (4) : 36～40 　 Journal of N anjin g A gricultural U niversity 受白叶枯病菌诱导的水稻抗病相关基因 rgi 97 的部分序列克隆 孔维文 , 邵敏 , 彭建令 , 董汉松 , 王金生 (南京农业大学农业部病虫监测与治理重点开放实验室 , 江苏 南京 210095) 摘要 : 利用 mRNA 差异显示技术从水稻中克隆到一个受白叶枯病菌诱导的基因片段 ( rgi 97) 。根据 RTPCR 结果 , rgi 97 具有诱导表达特性 , 在水稻受白叶枯病菌侵染后 24 h 时检测不到 , 36 h 时有微弱表达 , 而到 48 h 时表达增强。通过 3′RACE 技术获得了该片段 3′端约970 bp 的序列。相似性搜索表明 , 包含 3′末端的 rgi 97 可能的编码产物与拟南芥一个 未知蛋白有一定相似性 , 达 92 % , 提示该基因可能与水稻对白叶枯病的抗性有关。 关键词 : 水稻 ; 抗病性 ; 调控基因 中图分类号: S43242 　　　文献标识码 : A 　　　文章编号 : 1000 2030 (2003) 04 0036 05 Cloning of partial sequence of rgi 97 , a rice resistance associated gene induced by X anthomonas oryz ae pv. oryz ae KON G Weiwen , SHAO Min , PEN G Jianling , DON G Hansong , WAN G Jinsheng ( Key Laboratory of Monitoring and Management of Plant Diseases and Insects , Ministry of Agriculture , Nanjin g Agric Univ , Nanjin g 210095 , China) Abstract : Cloning and functional analysis of rice genes involved in pathogen resistance and induced by Xanthomonas oryz ae pv. oryz ae ( Xoo) is an important step towards understanding molecular mechanisms of rice resistance to Xoo . The 465 bp partial se quence of a gene , named rgi 97 , was obtained from rice by differential display of mRNA isolated from rice plants inoculated with Xoo . The rgi 97 was expressed in plants infected by bacteria but not in plants uninfected. Transcript of rgi 97 was not detected in plants of rice varieties IR24 and IRBB4 until 36 h after inoculation , and reached maxima