植物生长素合成酶iaaM基因在烟草韧皮部的特异表达对其发育的影响.PDF

38 1 () Vol.38 No.1 2012 2 Journal of Hunan Agricultural University (Natural Sciences) Feb ．2012 DOI:10.3724/SP.J.1238.00032 植物生长素合成酶 iaaM 基因在烟草韧皮部 的特异表达对其发育的影响 * ( 410128) 摘 要 (iaaM) () 关 键 词 中图分类号Q786 文献标志码A 文章编号1007− 1032(2012)01−0032−04 The effects of over expression of auxin synthesis gene iaaM in Nicotiana tabacum ’s Phloem * PENG Yan, WANG Ya-hong ,HUANG Li-hua, ZHANG Xue-wen (College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China) Abstract We constructed a plant phloem-specific expression iaaM vector to study the effect of IAA over-expression on phloem development. The vector was constructed by recombining the tryptophan monooxygenase gene (iaaM) with the phloem-specific promoter and the sucrose synthesis gene promoter of Arabidopsis thaliana. The recombinant was transformed into Nicotiana tabacum and several transgenic plants were screened out. Most of the transgenic plants showed abnormal morphologies as leave curling and delayed development and the seedlings grew slower compared to the wild control. The section observation of the stem showed that more secondary xylem and phloem cells were produced in transgenic plants compared to the wild-type. In addition, there were many adventitious roots abnormally grown out in the transgenic stems and numerous root hairs and lateral roots occurred in