红景天超微粉对小鼠急性肝损伤保护作用.doc

红景天超微粉对小鼠急性肝损伤保护作用 　 【摘要】 探讨红景天超微粉及乙醇提取物对小鼠急性肝损伤的保护作用，比较两者对肝损伤保护作用的差异.［ 方法 ］用D氨基半乳糖制作小鼠急性肝损伤动物模型，分别灌胃给予不同组小鼠相应剂型及剂量的红景天超微粉和乙醇提取物，连续4d，末次给药1h后测定小鼠血清丙氨酸转移酶(ALT)及天冬氨酸转氨酶(AST)含量，肝脏匀浆中丙二醛(MDA)含量及超氧化物歧化酶(SOD)活性，同时行肝组织切片，染色，观察病理变化.［结果］红景天超微粉和乙醇提取物均可明显降低急性肝损伤小鼠血清ALT，AST及肝组织匀浆中MDA含量，提高SOD活性.［结论］红景天超微粉和乙醇提取物对小鼠急性肝损伤均具有保护作用，而红景天超微粉的作用优于红景天乙醇提取物. 毕业论文 【关键词】 肝疾病 红景天 超微粉 小鼠 论文代写 ABSTRACT:OBJECTIVETo study the protective effect of the ultra fine powder and the ethanol extracts of Rhodiola sachalimemsis A.Bor for acute liver injury in mice，and compare the protective effect to dose effect relationship between equal dose of them.METHODS The mice performed by intragastric administration with the ultra fine powder and ethanol extract of Rhodiola sachalimemsis A.Bor, respectively. The mouse model of acute liver injury were induced by Dgalactosamine, and the serum levels of ALT and AST and the contents of MDA、activity of SOD in livers of the mice were measured, and pathologic changes were observed.RESULTSThe groups of ultra fine powder and ethanol extract of the Rhodiola sachalimemsis A.Bor significantly reduced the levels of serum ALT and AST which were induced by ethanol, and the contents of MDA,activity of SOD in livers were significant different compared to the model groups.CONCLUSIONThe ultra fine powder of the Rhodiola sachalimemsis A.Bor has protective effects against hepatic injury, and they were superior to ethanol extract. Key words:liver diseases；Rhodiola sacra;ultra fine powder；mice 论文代写 高山红景天（Rhodiola sachalimemsis A.Bor.）是景天科红景天属多年生草本植物，全草均可入药，具有清肺止咳及止血等功效，常用于 治疗 跌打损伤、烧烫伤、阳痿及糖尿病等. 研究 结果表明，红景天苷对实验性肝损伤小鼠具有较好的保护作用［1，2］，但尚未见红景天超微粉的相关报道.本实验观察了红景天超微粉对急性肝损伤小鼠的保护作用，并将其与红景天乙醇提取物的作用进行了比较. 1 材料与方法 1.1 材料 实验动物取雄性昆明种小鼠，体重为22～25g，由延边大学医学部实验动物科提供.高山红景天购自吉林省安图县药材市场，由延边大学药学院生药学教研部刘永镇教授鉴定为高山红景天（Rhodiola sachalimemsis A.Bor.）.D氨基半乳糖由重庆医科大学提供；护肝宁片为吉林省通化天立药业有限公司产品.丙氨酸转移酶(alanine aminotransferase， ALT)及天冬氨酸转氨酶(aspartate transaminase, AST)试剂盒均为北化康泰临床试剂有限公司产品；超