肝癌微环境中CD4+CD25+Treg细胞及肿瘤免疫细胞关系.docVIP

肝癌微环境中CD4+CD25+Treg细胞及肿瘤免疫细胞关系 　 作者：陈中 晏建军，倪家连，刘鲁岳，黄亮，严以群 【摘要】 ［目的］ 探讨肝癌微环境中Treg细胞的数量增多与HCC临床分期晚的可能原因。［方法］ 双重酶标免疫组织化学方法检测52例HCC组织中CD4+CD25+Treg细胞以及CD4+CD25-T（CD4+T）细胞分布，免疫组化EnVision法检测20例HCC组织中CD8+T细胞分布。［结果］ 正常肝脏组织中未发现Treg细胞，HCC组织中Treg细胞数量较癌旁组织明显增多（P=0.000），且肝癌组织中Treg细胞的数量与其浸润性CD4+T淋巴细胞的数量以及CD4+T/CD8+T比值呈显著负相关（r＝-0.539，P＝0.014；r＝-0.545，P＝0.000），而与浸润性CD8+T淋巴细胞的数量无明显相关性（r=-0.403，P=0.078）。［结论］ Treg细胞在体内可能通过细胞接触的方式抑制CD4+T 淋巴细胞的增殖来抑制肿瘤局部的免疫，去除或减少HCC微环境中浸润性Treg细胞数量可能提高肿瘤局部免疫治疗效果。 【关键词】 CD4+CD25+调节性T细胞 癌 肝细胞性 肿瘤浸润 淋巴细胞 Correlation between the Distribution of CD4+CD25+ Regulartory T(Treg) Cells and T-cell Infiltration in Microenvironment of Hepatocellular Carcinoma Abstract: ［Purpose］ To explore the causes of an increased prevalence of regulartory T(Treg) cells in microenvironment of hepatocellular carcinoma(HCC) which involved in TNM stage according to disease progression. ［Methods］ The CD4+CD25+ Treg and CD4+CD25-T (CD4+T) cells in the slides tissues of 52 cases with HCC were marked by Double-immunohistochemical, and CD8+T cells in the slides tissues of 20 cases with HCC by EnVision. ［Results］ There was no Treg cell in normal liver tissues. The number of Treg cells in the tissue of HCC was significantly more than that in the tissue of peri-cancer(P=0.000). The number of Treg cells in HCC tissue was negatively correlated to the ratio of CD4+T/CD8+T and CD4+T cells infiltration (r＝-0.539，P＝0.014；r＝-0.545，P＝0.000), however, with no correlation to CD8+T cells infiltration(r=-0.403,P=0.078). ［Conclusions］ Treg cells possibly suppress proliferation of CD4+T cells in a contact-dependent manner so that tumor cells can escape immune surveillance and result in invasion and progression. Functional deletion of tumor-infiltrating Treg cells in microenvironment of HCC can enhance tumor-specific immunotherapy. Key words: CD4+CD25+ regulartory T cell; hepatocellular carcinoma； tumor infiltrating；lymphocytes 肿瘤浸润淋巴细胞(TIL)是指位于原发和继发肿瘤细胞周围的淋巴细胞，主要由T 细胞组成。TIL具有杀伤肿瘤细胞、降低转移潜能的作用，因其与肿瘤细胞密切接