芦根多糖提取及其抗氧化活性探究.docVIP

芦根多糖提取及其抗氧化活性探究 　 作者：沈蔚， 任晓婷， 张建， 杨靖亚 【摘要】 　　目的利用微波法提取芦根多糖，测定其含量，并研究芦根多糖的抗氧化活性。方法通过正交实验探讨芦根多糖提取的最佳工艺，从清除抑制羟基自由基的产生、还原力和对脂质体抗氧化活性的测定3个方面研究了芦根多糖的体外抗氧化效果, 并同VC 进行比较。结果芦根多糖含量为0.798％。微波法提取芦根多糖的最佳工艺参数为料水比1∶3，微波强度为大火，提取时间8 min。多糖还原能力仅稍次于抗坏血酸；多糖对羟自由基的清除能力较抗坏血酸来说较弱；对脂质过氧化的抑制作用在吸光度小于等于0.88时与多糖的浓度成正相关。结论微波法对芦根中的多糖的提取有辅助作用，芦根多糖具有一定的抗氧化活性。 【关键词】 微波辅助提取； 芦根多糖； 提取工艺； 抗氧化 　　Abstract：ObjectiveTo study the antioxidant activity of Reed rhizome . MethodsThe prepration，purification and determination of polysaccharides in Reed rhizome were investigated．The optimal technical parameters of extracting polysaccharides from Reed rhizome were determined through orthogonal test．The antioxidative activity of Reed rhizome Polysaccharides were studied and compared with Vc including the experiment of inhibiting the formation of hydroxyl radical，reducing power and inhibiting formation of lipid peroxidation.ResultsThe result showed that the optimal technical parameters were liquid—solid ratio l：3 and the extracting time 8minutes under max power through microwave assistant extraction．The concentration of polysaccharide in Reed rhizome was 0.798％．Polysaccharides had obvious reducing capauty and was equal to Vc. Scavenging capability to hydroxyl radical of Polysaccharides was lower than Vc.When Abs was lower than 0.88,the inhibition to lipid peroxidation was equal to polysaccharide.ConclusionMicrowave method is helpful to extract the polysaccharides in the Reed rhizome, and it demonstrates the antioxidant activity of the polysaccharides. 　　Key words：Microwave assistant extraction； Polysaccharides in Reed rhizome； Extraction technology； Anti-oxidation 　　芦根是一种常用中药。据医书记载, 芦根性味甘、寒入肺、胃经。具有清热、生津、除烦、止呕、利尿等功效, 中医学称之为“火药”。现代药理分析，芦根含有薏苡素、天门冬酰胺等。它的药用价值除退热外, 还具有多种作用。芦根是一味具有多种功能的食材和中药材，它不仅在食用上具有多种搭配，而且在中医药学应用研究方面具有巨大的贡献［1］。 　　芦根别名芦茅根、苇根、芦柴根、芦菇根、顺江龙、水蓈蔃、芦通、苇子根、芦芽根、甜梗子，有清热生津、除烦、止呕、利尿的功能，主治热病烦渴、胃热呕吐、肺热咳嗽、肺痈吐脓、热淋涩痛。其来源为禾本科植物芦苇Phragmites communis Trin.的新鲜或干燥根茎。鲜芦根呈长圆柱形，有的略扁，长短不一，直径1～2 cm。表面黄白色，有光泽，外皮疏松可剥离，节呈环状，有残根及芽