原创力文档a suite of recombinant luminescent bacterial strains for the quantification of bioavailable heavy metals and toxicity testing一套重组发光细菌菌株的量化重金属生物有效性和毒性测试.pdfVIP
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a suite of recombinant luminescent bacterial strains for the quantification of bioavailable heavy metals and toxicity testing一套重组发光细菌菌株的量化重金属生物有效性和毒性测试
BMC Biotechnology BioMed Central
Research article Open Access
A suite of recombinant luminescent bacterial strains for the
quantification of bioavailable heavy metals and toxicity testing
Angela Ivask*, Taisia Rõlova and Anne Kahru
Address: Laboratory of Molecular Genetics, National Institute of Chemical Physics and Biophysics, Akadeemia tee 23, Tallinn, Estonia
Email: Angela Ivask* - angela@kbfi.ee; Taisia Rõlova - Taisia.Rolova@uku.fi; Anne Kahru - anne.kahru@kbfi.ee
* Corresponding author
Published: 8 May 2009 Received: 19 September 2008
Accepted: 8 May 2009
BMC Biotechnology 2009, 9:41 doi:10.1186/1472-6750-9-41
This article is available from: /1472-6750/9/41
© 2009 Ivask et al; licensee BioMed Central Ltd.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0),
which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Background: Recombinant whole-cell sensors have already proven useful in the assessment of the
bioavailability of environmental pollutants like heavy metals and organic compounds. In this work
19 recombinant bacterial strains representing various Gram-positive (Staphylococcus aureus and
Bacillus subtilis) and Gram-negative (Escherichia coli, Pseudomonas fluorescens) bacteria were
constructed to express the luminescence encoding genes luxCDABE (from Photorhabdus
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