原创力文档a system for precise analysis of transcription-regulating elements of immunoglobulin genestranscription-regulating元素的系统进行精确分析的免疫球蛋白基因.pdfVIP
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a system for precise analysis of transcription-regulating elements of immunoglobulin genestranscription-regulating元素的系统进行精确分析的免疫球蛋白基因
BMC Biotechnology BioMed Central
Methodology article Open Access
A system for precise analysis of transcription-regulating elements of
immunoglobulin genes
1 1 1 1,2
Emily Y Cheng , Cathy Collins , Maribel Berru and Marc J Shulman*
Address: 1Department of Immunology, University of Toronto, Toronto, Canada and 2Department of Molecular and Medical Genetics, University
of Toronto, Toronto, Canada
Email: Emily Y Cheng - emily.cheng@utoronto.ca; Cathy Collins - cathy.collins@utoronto.ca; Maribel Berru - maribel.berru@utoronto.ca;
Marc J Shulman* - marc.shulman@utoronto.ca
* Corresponding author
Published: 04 October 2005 Received: 08 June 2005
Accepted: 04 October 2005
BMC Biotechnology 2005, 5:27 doi:10.1186/1472-6750-5-27
This article is available from: /1472-6750/5/27
© 2005 Cheng et al; licensee BioMed Central Ltd.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0),
which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Background: Precise analysis of expression-regulating elements, such as enhancers and insulators,
requires that they be tested under reproducible, isogenic conditions. The commonly used methods
of transfecting DNA into cell lines and selecting for drug resistance lack the requisite precision, as
they yield cell lines in which vary
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