an algorithm for automatic evaluation of the spot quality in two-color dna microarray experiments一个算法自动评估现场质量的双色dna微阵列实验.pdfVIP

BMC Bioinformatics BioMed Central Methodology article Open Access An algorithm for automatic evaluation of the spot quality in two-color DNA microarray experiments Eugene Novikov* and Emmanuel Barillot Address: Service Bioinformatique, Institut Curie, 26 Rue dUlm, 75248 Paris Cedex 05, France. Email: Eugene Novikov* - Eugene.Novikov@curie.fr; Emmanuel Barillot - Emmanuel.Barillot@curie.fr * Corresponding author Published: 09 December 2005 Received: 17 June 2005 Accepted: 09 December 2005 BMC Bioinformatics 2005, 6:293 doi:10.1186/1471-2105-6-293 This article is available from: /1471-2105/6/293 © 2005 Novikov and Barillot; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Background: Although DNA microarray technologies are very powerful for the simultaneous quantitative characterization of thousands of genes, the quality of the obtained experimental data is often far from ideal. The measured microarrays images represent a regular collection of spots, and the intensity of light at each spot is proportional to the DNA copy number or to the expression level of the gene whose DNA clone is spotted. Spot quality control is an essential part of microarray image analysis, which must be carried out at the level of individua