an improved microtiter assay for evaluating anti-hiv-1 neutralizing antibodies from sera or plasma一种改进的微量滴定分析评估anti-hiv-1中和抗体血清或血浆.pdfVIP

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an improved microtiter assay for evaluating anti-hiv-1 neutralizing antibodies from sera or plasma一种改进的微量滴定分析评估anti-hiv-1中和抗体血清或血浆.pdf

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an improved microtiter assay for evaluating anti-hiv-1 neutralizing antibodies from sera or plasma一种改进的微量滴定分析评估anti-hiv-1中和抗体血清或血浆

BMC Infectious Diseases BioMed Central Technical advance Open Access An improved microtiter assay for evaluating anti-HIV-1 neutralizing antibodies from sera or plasma 1,2 1 1,3 Chiyu Zhang , Yunyun Chen and Kunlong Ben* Address: 1Laboratory for Molecular and Cell Immunology, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, China, 2The School of Medical Technology, Jiangsu University, Zhenjiang, Jiangsu 212001, China and 3Kunming Chinawave Biotechnology Company Ltd., Kunming, Yunnan 650106, China Email: Chiyu Zhang - zhangcy1999@; Yunyun Chen - chenyy02@; Kunlong Ben* - kben@ * Corresponding author Published: 23 December 2003 Received: 05 October 2003 Accepted: 23 December 2003 BMC Infectious Diseases 2003, 3:30 This article is available from: /1471-2334/3/30 © 2003 Zhang et al; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the articles original URL. HIV-1neutralizing antibodyMTTmicrotiter neutralization assayserum or plasma Abstract Background: The anti-HIV-1 neutralizing antibody assay is widely used in AIDS vaccine research and other experimental and clinical studies. The vital dye staining method applied in the detection of anti-HIV-1 neutralizing antibody has been used in many laboratories. However, the unknown factor(s) in sera