analysis of escherichia coli o157 clinical isolates by multilocus sequence typing分析大肠杆菌o157临床分离株的茎序列类型.pdfVIP

Rajkhowa et al. BMC Research Notes 2010, 3:343 /1756-0500/3/343 CORRESPONDENCE Open Access Analysis of Escherichia coli O157 clinical isolates by multilocus sequence typing 1 1 2 2 1 1* Swaraj Rajkhowa , Joy Scaria , Daniel L Garcia , Kimberlee A Musser , Bruce L Akey , Yung-Fu Chang Abstract Background: Although many strain typing methods exist for pathogenic Escherichia coli, most have drawbacks in terms of resolving power, interpretability, or scalability. For this reason, multilocus sequence typing (MLST) is an appealing alternative especially when applied to the typing of temporal and spatially separated isolates. This method relies on an unambiguous DNA sequence analysis of nucleotide polymorphisms in housekeeping genes and has shown a high degree of intraspecies discriminatory power for bacterial and fungal pathogens. Results: Here we used the MLST method to study the genetic diversity among E. coli O157 isolates collected from humans from two different locations of USA over a period of several years (2000-2008). MLST analysis of 33 E. coli O157 patient isolates using the eBurst algorithm distinguished 26 different sequence types (STs), which were clustered into two clonal groups and 11 singletons. The predominant ST was ST2, which consisted of 5 isolates (14.28%) followed by ST1 (11.42%). All the isolates under clonal group I exhibited a virtually similar virulence profile except for two strains, which tested negative for the presence of stx genes. The isolates that were assigned to clonal group II in addition to the 11 singletons were found to be phylogenetically distant from clonal group I. Furthermore, we observed a positive correlation between the virulence profile of the isolates and their