an initial comparative map of copy number variations in the goat (capra hircus) genome初步比较映射拷贝数变化的山羊(·卡普拉狐臭)基因组.pdfVIP

Fontanesi et al. BMC Genomics 2010, 11:639 /1471-2164/11/639 RESEARCH ARTICLE Open Access An initial comparative map of copy number variations in the goat (Capra hircus) genome 1* 2 1,3 3 1 1 Luca Fontanesi , Pier Luigi Martelli , Francesca Beretti , Valentina Riggio , Stefania Dall’Olio , Michela Colombo , 2 1 3 Rita Casadio , Vincenzo Russo , Baldassare Portolano Abstract Background: The goat (Capra hircus) represents one of the most important farm animal species. It is reared in all continents with an estimated world population of about 800 million of animals. Despite its importance, studies on the goat genome are still in their infancy compared to those in other farm animal species. Comparative mapping between cattle and goat showed only a few rearrangements in agreement with the similarity of chromosome banding. We carried out a cross species cattle-goat array comparative genome hybridization (aCGH) experiment in order to identify copy number variations (CNVs) in the goat genome analysing animals of different breeds (Saanen, Camosciata delle Alpi, Girgentana, and Murciano-Granadina) using a tiling oligonucleotide array with ~385,000 probes designed on the bovine genome. Results: We identified a total of 161 CNVs (an average of 17.9 CNVs per goat), with the largest number in the Saanen breed and the lowest in the Camosciata delle Alpi goat. By aggregating overlapping CNVs identified in different animals we determined CNV regions (CNVRs): on the whole, we identified 127 CNVRs covering about 11.47 Mb of the virtual goat genome referred to the bovine genome (0.435% of the latter genome). These 127 CNVRs included 86 loss