assessment of phytochemical content, polyphenolic composition, antioxidant and antibacterial activities of leguminosae medicinal plants in peninsular malaysia评估植物化学的内容,多酚成分、抗氧化和抗菌活性的豆科药用植物在马来西亚半岛.pdfVIP
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assessment of phytochemical content, polyphenolic composition, antioxidant and antibacterial activities of leguminosae medicinal plants in peninsular malaysia评估植物化学的内容,多酚成分、抗氧化和抗菌活性的豆科药用植物在马来西亚半岛
Chew et al. BMC Complementary and Alternative Medicine 2011, 11:12
/1472-6882/11/12
RESEARCH ARTICLE Open Access
Assessment of phytochemical content,
polyphenolic composition, antioxidant and
antibacterial activities of Leguminosae medicinal
plants in Peninsular Malaysia
1 1 1 1 2,3 1*
Yik Ling Chew , Elaine Wan Ling Chan , Pei Ling Tan , Yau Yan Lim , Johnson Stanslas , Joo Kheng Goh
Abstract
Background: Many medicinal plants from Leguminosae family can be found easily in Malaysia. These plants have
been used as traditional medicines by local ethnic groups, where they are prepared as decoction, pastes for
wound infections, and some have been eaten as salad. This paper focused on the assessment of antioxidant
potential, antibacterial activity and classes of phytochemicals of nine plants from the Leguminosae family.
Methods: Acacia auriculiformis, Bauhinia kockiana, Bauhinia purpurea, Caesalpinia pulcherrima, Calliandra tergemina,
Cassia surattensis, Leucaena leucocephala, Peltophorum pterocarpum, and Samanea saman were extracted with
aqueous methanol and dichloromethane:methanol mixture to test for antioxidant and antibacterial activities. The
Folin-Ciocalteu assay was conducted to quantify the total phenolic content and 2, 2-diphenyl-1-picrylhydrazyl
(DPPH) assay was used to determine the free radical quenching capacity. Antibacterial activity was assessed using
disc diffusion (Kirby-Bauer) assay. Screening for major classes of phytochemical was done using standard chemical
tests.
Results: B. kockiana flowers and C. pulcherrima leaves contained high total phenolic content (TPC) and strong DPPH
radical scavenging ability with TPC of 8280 ± 498 mg GAE/100 g, IC50 of 27.0 ± 5.0
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