association of mutation patterns in gyrab genes and ofloxacin resistance levels in mycobacterium tuberculosis isolates from east china in 2009协会的突变模式gyrab基因和氧氟沙星阻力位在2009年从中国东部结核分枝杆菌分离株.pdfVIP

Cui et al. BMC Infectious Diseases 2011, 11:78 /1471-2334/11/78 RESEARCH ARTICLE Open Access Association of mutation patterns in gyrA/B genes and ofloxacin resistance levels in Mycobacterium tuberculosis isolates from East China in 2009 * Zhenling Cui , Jie Wang, Junmei Lu, Xiaochen Huang and Zhongyi Hu Abstract Background: This study aimed to analyze the association of mutation patterns in gyrA and gyrB genes and the ofloxacin resistance levels in clinical Mycobacterium tuberculosis isolates sampled in 2009 from East China. Methods: The quinolone resistance-determining region of gyrA/B were sequenced in 192 M. tuberculosis clinical isolates and the minimal inhibitory concentrations (MICs) of 95 ofloxacin-resistant M. tuberculosis isolates were determined by using microplate nitrate reductase assays. Results: Mutations in gyrA (codons 90, 91 and 94) and in gyrB (G551R, D500N, T539N, R485C/L) were observed in 89.5% (85/95) and 11.6% (11/95) of ofloxacin-resistant strains, respectively. The gyrB mutations G551R and G549D were observed in 4.1% (4/97) of ofloxacin-susceptible strains and no mutation was found in gyrA in ofloxacin- susceptible strains. The MICs of all ofloxacin-resistant strains showed no significant difference among strains with mutations at codons 90, 91 or 94 in gyrA (F = 1.268, p = 0.287). No differences were detected among strains with different amino acid mutations in the quinolone resistance-determining region of gyrA (F = 1.877, p = 0.123). The difference in MICs between ofloxacin-resistant strains with mutations in gyrA only and ofloxacin-resistant strains with mutations in both gyrA and gyrB genes was not statistically significant (F = 0.549, p = 0.461). Conclusions: Although gyrA/B mutations can lead to ofloxacin resistance in M. tuberculosis, there were no ass