ovarian cyst fluid is a rich proteome resource for detection of new tumor biomarkers卵巢囊肿液是一个富有的资源发现新的肿瘤标记物的蛋白质组.pdfVIP

Kristjansdottir et al. Clinical Proteomics 2012, 9:14 /content/9/1/14 CLINICAL PROTEOMICS RESEARCH Open Access Ovarian cyst fluid is a rich proteome resource for detection of new tumor biomarkers 1* 1 2 1 2 1 Björg Kristjansdottir , Karolina Partheen , Eric T Fung , Janusz Marcickiewicz , Christine Yip , Mats Brännström and Karin Sundfeldt1 Abstract Background: We aimed to investigate the use of ovarian cyst fluid as a source for biomarker discovery and to find novel biomarkers for use in the diagnosis of epithelial ovarian tumors. Results: Ovarian cyst fluids from 218 women were collected and 192 (benign n = 129, malignant n = 63) were analyzed using mass spectrometry. 1180 peaks were detected, 221 of which were differently expressed between benign and malignant ovarian tumors. Seventeen peaks had receiver operating curve and area under the curve values 0.70; the majority of these represented peaks for apolipoproteins C-III and C-I (ApoC-I), transthyretin (TTR), serum amyloid A4 (SAA4), and protein C inhibitor (PCI). ApoC-III, PCI, and serum CA125, with an ROC AUC 0.94 was the best combination for diagnosing epithelial ovarian cancer. ApoC-III and PCI was analyzed with ELISA in the original cohort (n = 40) and in 40 new cyst fluid samples for confirmation with an independent method and validation. Results from MS and ELISA for ApoC-III correlated well (p = 0.04). In the validation set, ApoC-III was significantly (p = 0.001) increased in the malignant epithelial ovarian cancers. Conclusions: Fluid fr