preparative isolation and purification of four compounds from cistanches deserticola y.c. ma by high-speed counter-current chromatography四个化合物的制备分离和纯化从cistanches酶类y.c.马高速逆流色谱法.pdf

Molecules 2012, 17, 8276-8284; doi:10.3390/molecule OPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Article Preparative Isolation and Purification of Four Compounds from Cistanches deserticola Y.C. Ma by High-Speed Counter-Current Chromatography Lifeng Han 1,2, Lina Ji 1, Mavis Boakye-Yiadom 2, Wei Li 2,3, Xinbo Song 2 and Xiumei Gao 1,* 1 Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, 312 Anshanxi Road, Tianjin 300193, China 2 Tianjin Key Laboratory of TCM Chemistry and Analysis, Tianjin University of Traditional Chinese Medicine, 312 Anshanxi Road, Tianjin 300193, China 3 Inner Mongolia Mandela Sand Industry Development Co., Ltd., Left Banner of Alashan, Inner Mongolia 750300, China * Author to whom correspondence should be addressed; E-Mail: gaoxiumei@. Received: 23 May 2012; in revised form: 28 June 2012 / Accepted: 3 July 2012 / Published: 10 July 2012 Abstract: Following a constituent enrichment step on a silica gel column, four phenyl-ethanoid glycosides were successfully isolated from Cistanches deserticola and purified by preparative high-speed counter-current chromatography (HSCCC) with a two-phase solvent system composed of ethyl acetate-n-butanol-ethanol-water (40:6:6:50, v/v/v/v). A total of 30.9 mg acteoside, 13.0 mg isoacteoside, 12.5 mg syringalide A 3-α-L-rhamnopyranoside and 7.2 mg 2-acetylacteoside with purity of higher than 95%, as determined by HPLC-ELSD,