第08章 分光光度法测定红发夫酵母中虾青素含量.pdfVIP

第29 卷第2期 Vol.29 No.2 2001 年6 月 JOURNAL OFZ EJIANG UNIVERSITY OFTEC NOLOGY June 2001 : 1006-4303(2001)02-0120-04 , , ( , 310032) : 建立了测定红发夫酵母胞内虾青素含量的前处理工艺及分光光度分析方法其前处 理工艺 次提取率达96.2%,提取液在480 nm 处有最大吸收, 建立的分光光度法加标回收 率为100.2%~ 104. 1%,标准偏差为0.21%~ 0.43%,表明该分析方法准确性高,重复性好, 且简便快速, 可以作为菌种筛选发酵条件优化过程中快速测定菌体中虾青素含量的方 法 : 分光光度法; 红发夫酵母; 虾青素 :Q815;O657.3 :A Analysis of astaxathin from p haff ia rhodozyma by spectrophotometry XU Pe-i ya, Z ENG Yu-guo, S EN Yin-chu (College of Biological Environmental Engineering, Zhejiang University of Technology, angzhou 310032, China) Abstract: A technique of treatment before assay and an analytical assay of astaxathin were designed fromp haff ia rhodozyma by spectrophotometry. The percentage of extraction reached 96. 2% in the technique of treatment beforeassay. Themaximum absorption of the solution lay is480nm. Therecov- ery is 100.2%~ 104. 1%; and the standard deviation is 0.21%~ 0. 43%. The methed is simple and applicabletoanalysis of astaxathin fromp haff ia rhodozyma in thefiltration of mutant strain andthe optimization of fermentation condition. Key words: spectrophotometry; p haff ia rhodozyma; astaxathin 0 (3,3c- - 4,4c- - B,Bc- ), , , [ 1] ,, , : ,, : 2000- 10- 17; :2001- 02- 18 : (2000268) : ( 1964- ) , , , , 2 , : # 121 # , , , , :() () , ; ,,;, ;