production of viable hybrids in salmonids by triploidization生产可行的混合动力车在triploidization大马哈鱼.pdfVIP

Production of viable hybrids in salmonids by triploidization B. CHEVASSUS, R. GUYOMARD, D. CHOURROUT Edwige QUILLET with the technical assistance of G. BURGER and A. EVAUXD LN.R.A., Laboratoire de Physiologie des Poissons, F 78350 Jouy-en-Josas r C.N.E.x.O., Centre Oceanologie de Bretagne, F 29273 Brest exCed Summary Rainbow trout eggs were fertilized with milt collected from various salmanids (brown trout Salmo trutta ; brook trout Salvelinus lontinaliv ; coho salmon Oncorhynchus kisutch) and heat-shocked few minutes later in order to prevent the second polar body extrusion. This operation resulted in high survival rates after 161 days : respectively 49.2 p. 100, 66.2 p. 100 and 12.5 p. 100 of the diploid rainbow trout control (basis 100 p. 100) ; the triploid hybrid constitution of these fish was ascertained by karyological and biochemical investigations. These data contrast with the bad yields of the corresponding diploid hybridizations (no heat shock) respectively 0 p. 100, 12.3 p. 100 and 0 p. 100. Such triploid hybrid salmonids, that are easy to produce, could be sterile and conse- quently of a great interest in aquaculture, where fish reproduction often makes the profi- tability decrease. words : salmonids. Key Hybrids, triploidy, Résumé Production d’hybrides viables chez les salmonidés par triploïdisation Des ovules de truite arc-en-ciel Salnxo gairdneri ont été inséminés par du sperme de divers salmonidés (truite fario Salino trutta; omble de fontaine Salvelinus fontinalis; saumon coho Oncorhynchus