protein profiling reveals inter-individual protein homogeneity of arachnoid cyst fluid and high qualitative similarity to cerebrospinal fluid蛋白质分析揭示了inter-individual蛛网膜囊肿液和高的同质性定性相似脑脊液.pdfVIP

Berle et al. Fluids and Barriers of the CNS 2011, 8:19 FLUIDS AND BARRIERS /content/8/1/19 OF THE CNS RESEARCH Open Access Protein profiling reveals inter-individual protein homogeneity of arachnoid cyst fluid and high qualitative similarity to cerebrospinal fluid 1* 1,2 3 2 2 4 Magnus Berle , Ann C Kroksveen , Øystein A Haaland , Thin T Aye , Jill A Opsahl , Eystein Oveland , Knut Wester5,6, Rune J Ulvik1,7, Christian A Helland5,6 and Frode S Berven2,8 Abstract Background: The mechanisms behind formation and filling of intracranial arachnoid cysts (AC) are poorly understood. The aim of this study was to evaluate AC fluid by proteomics to gain further knowledge about ACs. Two goals were set: 1) Comparison of AC fluid from individual patients to determine whether or not temporal AC is a homogenous condition; and 2) Evaluate the protein content of a pool of AC fluid from several patients and qualitatively compare this with published protein lists of cerebrospinal fluid (CSF) and plasma. Methods: AC fluid from 15 patients with temporal AC was included in this study. In the AC protein comparison experiment, AC fluid from 14 patients was digested, analyzed by LC-MS/MS using a semi-quantitative label-free approach and the data were compared by principal component analysis (PCA) to gain knowledge of protein homogeneity of AC. In the AC proteome evaluation experiment, AC fluid from 11 patients was pooled, digested, and fractionated by SCX chromatography prior to analysis by LC