原创力文档rapid chromosome territory relocation by nuclear motor activity in response to serum removal in primary human fibroblasts快速的染色体区域搬迁由核运动活动除血清反应主要人类成纤维细胞.pdfVIP
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rapid chromosome territory relocation by nuclear motor activity in response to serum removal in primary human fibroblasts快速的染色体区域搬迁由核运动活动除血清反应主要人类成纤维细胞
Mehta et al. Genome Biology 2010, 11:R5
/2010/11/1/R5
R E S E A R C H Open Access
Research
Rapid chromosome territory relocation by nuclear
motor activity in response to serum removal in
primary human fibroblasts
1,2 2 1
Ishita S Mehta1, Manelle Amira , Amanda J Harvey and Joanna M Bridger*
Chromosome positioning dynamicschromosome territories occurs within 15 min-utes of serum starvatNuclear myosin 1β-dependent repositioning ofion in human cells.
Abstract
Background: Radial chromosome positioning in interphase nuclei is nonrandom and can alter according to
developmental, differentiation, proliferation, or disease status. However, it is not yet clear when and how chromosome
repositioning is elicited.
Results: By investigating the positioning of all human chromosomes in primary fibroblasts that have left the
proliferative cell cycle, we have demonstrated that in cells made quiescent by reversible growth arrest, chromosome
positioning is altered considerably. We found that with the removal of serum from the culture medium, chromosome
repositioning took less than 15 minutes, required energy and was inhibited by drugs affecting the polymerization of
myosin and actin. We also observed that when cells became quiescent, the nuclear distri bution of nuclear myosin 1β
was dramatically different from that in proliferating cells. If we suppressed the expression of nuclear myosin 1β by using
RNA-interference procedures, the movement of chromosomes after 15 minutes in low serum was inhibited. When high
serum was restored to the serum-starved cultures, chromosome repositioning was evident only after 24 to 36 hours,
and this coincided with a r
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