resistance loci affecting distinct stages of fungal pathogenesis use of introgression lines for qtl mapping and characterization in the maize - setosphaeria turcica pathosystem抗真菌致病位点影响不同阶段使用基因渗入qtl定位和描述在玉米- setosphaeria turcica pathosystem.pdfVIP

Chung et al. BMC Plant Biology 2010, 10:103 /1471-2229/10/103 R E S E A R C H A R T I C L E Open Access Research article Resistance loci affecting distinct stages of fungal pathogenesis: use of introgression lines for QTL mapping and characterization in the maize - Setosphaeria turcica pathosystem 1 1 1 3 4 5 Chia-Lin Chung , Joy M Longfellow , Ellie K Walsh , Zura Kerdieh , George Van Esbroeck , Peter Balint-Kurti and Rebecca J Nelson*1,2 Abstract Background: Studies on host-pathogen interactions in a range of pathosystems have revealed an array of mechanisms by which plants reduce the efficiency of pathogenesis. While R-gene mediated resistance confers highly effective defense responses against pathogen invasion, quantitative resistance is associated with intermediate levels of resistance that reduces disease progress. To test the hypothesis that specific loci affect distinct stages of fungal pathogenesis, a set of maize introgression lines was used for mapping and characterization of quantitative trait loci (QTL) conditioning resistance to Setosphaeria turcica, the causal agent of northern leaf blight (NLB). To better understand the nature of quantitative resistance, the identified QTL were further tested for three secondary hypotheses: (1) that disease QTL differ by host developmental stage; (2) that their performance changes across environments; and (3) that they condition broad-spectrum resistance. Results: Among a set of 82 introgression lines, seven lines were confirmed as more resistant or susceptible than B73. Two NLB QTL were validated in BC4 F2 segregating populations and advanced introgression lines. These loci, designated qNLB1.02 and qNLB1.06, were investigated in detail by comparing th