20120113低毒病毒CHV1-CN280生防潜力的初步研究.pdfVIP

28(1)80-86 中国生物防治学报 Chinese Journal of Biological Control 2012 年 2 月 低毒病毒CHV1-CN280生防潜力的初步研究 张林巧，高 坤，邓清超，叶 云，王克荣* (南京农业大学植物保护学院，南京 210095) 摘要：前期研究表明，分离自中国的低毒病毒CHV1-CN280 与其它多数低毒病毒相比具有更高的水平传播 能力。本研究中，我们对CHV1-CN280 的田间定殖能力及其它生防性状进行了评估。与其它两个模式低毒病 毒相比，CHV1-CN280 对栗疫菌Cryphonectria parasitica 的毒力显著弱于典型的烈性低毒病毒CHV1-EP713 ， 而更接近于典型的温和低毒病毒CHV1-Euro7 。强致病性的栗疫菌感染CHV1-CN280 后，成为典型的低毒菌 株，不再对板栗植株造成严重危害，但是与感染典型烈性低毒病毒 CHV1-EP713 的菌株相比，寄主真菌在 板栗枝条上仍保持了一定的定殖能力，其菌丝生长速率和分生孢子产量较高。初步的田间试验表明，在人 工接种两年后，CHV1-CN280 在田间成功定殖，并已扩散到田间自然存在的、与人为释放的低毒菌株不亲 和的其它营养体亲合群的栗疫菌菌株中。结果表明，低毒病毒CHV1-CN280 对栗疫病具有非常好的生物防 治潜力。 关 键 词：栗疫菌；低毒病毒；生物防治 中图分类号：S436.64; S476 文献标识码：A 文章编号：1005-9261 (2012)01-0080-07 Biological Control Potential of the Hypovirus CHV1-CN280 against Chestnut Blight * ZHANG Linqiao, GAO Kun, DENG Qingchao, YE Yun, WANG Kerong (College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China) Abstract: The previous studies showed that the hypovirus CHV1-CN280 isolated from North China had higher horizontal transmission abilities than many other CHV1 (Cryphonectria hypovirus 1) stains. In this paper, colonization capability of CHV1- CN280 in field and other biological characteristics were investigated. In comparison in the virulence with the other two well studied hypoviruses, CHV1-CN280 is more close to the mild virus strain CHV1-Euro7 than to the severe virus strain CHV1-EP713. Highly virulent C. parasitica strains became into lower virulent ones after infection with CHV1-CN280. The infected ones were able to colonize chestnut stem tissue and had higher levels of mycelial growth rate and asexual sporulation than strains