牛催乳素受体基因遗传多态性及其与部分精液品质-吉林农业大学学报.pdf

吉林农业大学学报 2010, 32( 2) : 195~ 199, 208 http / / x uebao.j lau. ed u. cn Journal of Jilin Agricultural University Email j lndxb@ vip . sina. com 1 2 1 1 3 1 宋永利, 刘 畅, 秦丽红, 张金玉 , 赵玉民, 赵志辉 1. 吉林大学畜牧兽医学院, 长春 130062; 2. 山东省农业科学院, 济南250100; 3. 吉林省农业科 学院, 公主岭136100 : 采用PCR- SSCP 方法对种公牛群体的催乳素受体基 ( PRLR) 所有外显子及 5UTR 多态性进行检 测结果表明: 外显子1及外显子8 分别检测到了2 种等位基 AB 和CD, 其中AC 为群体中优势基 , 其 他外显子及5UTR 没有遗传多态性; 相关性分析表明, 第1外显子位点AA 型鲜精顶体完整率显著高于AB 型 ( P = 0012) , 第8 外显子位点CD 型冻精活力显著高于CC 型和DD 型( P = 0004) 初步推断PRLR 为影响种 公牛繁殖性状的一个候选基 : 种公牛; 催乳素受体; 生产性状 : S8233 : A : 2010) SNP Analysis of Prolactin Receptor Gene and Its Relationship with Se men Quality in Bulls 1 2 1 1 3 1 SONG Yongli , LIU Cha ng , QIN Lihong , ZHAGN Jiny u , ZHAO Y umin , ZHAO Zhihui 1. College fo A nimal Science and Veterinary , Jilin University , Chang chun 130062, China ; 2. Shan dong Academy of Agricultural Sciences, Jinan 250100, China; 3. Jilin Academy fo Agricultural Sciences , Gongzhuling 136100, China Abstract: The exo s a d 5UTR polymorphisms of PRLR ge e i bulls were detected i this reserch by PCR- SSCP. The result showed that alleles A, B i exo 1 a d C, D i exo 8were detected i this pop ulatio , amo g which A a d C domi ated, a d there was o polymorphism i other exo s a d 5UTR; The relatio ship betwee ge etypes a d performa ceswas a alyzed, a d the results i dicated that fresh ac i tegrity rate of ge otype AA was higher tha ge otype AB i exo 1 a d froze seme e ergy of ge otype CD was much higher tha ge otype