鸡源益生菌jm-11菌株的胃肠道耐受及其抗腹泻的研究-中国农学通报.pdf

中国农学通报 2011,27(7):338-342 Chinese Agricultural Science Bulletin 鸡源益生菌JM-11菌株的胃肠道耐受及其抗腹泻的研究 郝庆红，郭云霞，吴国江，王世英，朱宝成 （河北农业大学生命科学学院，河北保定071001） 摘 要：试验从健康的鸡盲肠内容物中筛选出具有较高抑菌活性的病源拮抗菌JM- 11 菌株，经形态、生理 生化及16S rDNA 序列鉴定此菌为枯草芽孢杆菌（Bacillus subtilis subsp . subtilis）。经耐受胃肠道环境及 胆盐试验，结果表明，JM- 11 菌株能耐受0.3% 的胆盐环境，在pH 为3.0 的人工胃液中能够很好的存活。 利用抗生素打乱雏鸡肠道菌群后，饲喂致病性大肠杆菌来制作腹泻模型。经益生菌JM- 11对腹泻雏鸡 的治疗试验结果显示，JM- 11 能显著降低腹泻雏鸡粪便中的大肠杆菌的数量（P0.01 ），增加乳酸菌的数 量（P0.01 ），提高雏鸡免疫器官指数（P0.05 ），对雏鸡具有安全无毒的作用。 关键词：鸡；抗腹泻；益生菌；耐受性 中图分类号：S831 文献标志码：A 论文编号：2010-2982 TheToleranceinGastrointestinalTractEnvironmentandAnti-diarrheaofStrainJM-11 fromChickenAgainstPathogenicBacteria Hao Qinghong, Guo Yunxia, Wu Guojiang, Wang Shiying, Zhu Baocheng （CollegeofLifeScience, AgricultureUniversityofHebei, Baoding Hebei 071001） Abstract: In this study, strain JM-11 with antagonistic activity against E. coli was isolated from the cecum of healthy chicken. As the morphology characteristics and physiological and biochemical characteristics of the strain JM-11 was finally identified as Bacillus subtilis subsp . subtilis. The test result of the tolerance in gastrointestinal tract and Bile salts were showed as follows: the strain JM-11 could survive in the simulated natural (bile salts content 0.3% , pH 3.0 artificial gastric juice) environment. The model of diarrhea was produced by using antibiotics to break flora balance of intestinal tract, then pathogenicity E.coli was fed. The result of curing diarrhea of chicken by strain JM- 11 was showed as follows: the quantity of E.coli was remarkably decreased (P0.01), Lactobacilli activities significantly increased (P0.01), immune