ntc3h基因对烟草类黄酮及绿原酸合成的影响-中国烟草科学.pdfVIP

8 Chinese Tobacco Science 2016-0237 1 NtC3H 1 2 2 1 1 1* 1. 271018 2. 157100 NtC3H pCXSN::NtC3H NtC3H HPLC 3.6 6.1 24.6 NtC3H NtC3H ：S572.03 1007-5119201601-0008-06 DOI 10.13496/j.issn.1007-5119.2016.01.002 The Influence of NtC3H on the Synthesis of Flavonoids and Chlorogenic Acid in Tobacco 1 2 2 1 1 1* LI Yang , TANG Xuebing , LI Xiaofeng , LI Ming , CHU Zhaohui , DING Xinhua （1. State Key Laboratory of Crop Biology, Shandong Provincial Key Laboratory of Agricultural Microbiology, Shandong Agricultural University, Taian, Shandong 271018, China; 2. Heilongjiang Tobacco Industry Co., Ltd., Hailin Cigarette Factory, Hailin, Heilongjiang 157100, China ） Abstract: To test whether NtC3H participates in the biosynthesis of flavonoids, and chlorogenic acid (CGA) in tobacco and increases the contents of these secondary metabolites, we constructed the over-expression vector pCXSN::NtC3H and introduced it into Nicotiana tabacum var. samsun via Agrobacterium -mediated transformation. The concentrations of the different secondary metabolites including CGA, rutin, and kaempferol rutinoside in leaves of wild-type and transgenic lines were determined by HPLC. Compared to wild-type samsun tobacco, in transgenic tobacco the concentrations of CGA were increased 3.6-fold, rutin and kaempferol rutinoside were increased 6.1- and 24.6-fold respectively. These results suggested that NtC3H positively regulated the synthesis of flavonoids and CGA in tobacco. Keywords: tobacco; N