牛尾毛中稳定碳同位素组成变化规律研究.doc

牛尾毛中稳定性碳同位素组成变化规律研究 郭波莉，魏益民，潘家荣 （中国农业科学院农产品加工研究所/农业部农业核技术与农产品加工重点实验室，北京 100094） 摘要：【目的】研究牛尾毛中稳定性碳同位素组成随饲料改变的变化规律，确证牛尾毛一旦长成，其中的稳定性碳同位素不再与其它部分的进行交换，每段毛发记录的同位素信息可反映毛发生长时的饲料信息。【方法】设计控制饲料配比的牛模型试验，利用同位素比率质谱仪测定不同段位牛尾毛中的δ13C值，分析不同段位牛尾毛的δ13C值随饲料改变的变化趋势，以及牛尾毛中的δ13C值与饲料、牛个体的关系。【结果】随着牛生育过程中饲料成分的改变，不同段位牛尾毛中碳同位素组成有极显著差异，而且饲料对牛尾毛中碳同位素组成的影响远大于个体对其的影响；分段取得的牛尾毛δ13C值与其饲料δ13C值、C4植物饲料比例呈极显著相关（P＜0.01），而不分段的牛尾毛δ13C值与饲料碳同位素组成相关性不显著。【结论】牛尾毛一旦长成，其中的稳定性碳同位素不再与其它部分进行交换，每段毛发的同位素信息可反映牛在毛发生长时的饲料信息，牛尾毛可以看作是记录牛饲料改变信息的档案库。 关键词：牛；牛尾毛；追溯；稳定性碳同位素 Study on the Changing Law of Stable Carbon Isotope Composition in Cattle Tail Hair GUO Bo-li, WEI Yi-min, PAN Jia-rong (Key Laboratory of Agricultural Nuclear Technology and Agro-Food Processing, Ministry of Agriculture/Institute of Agro-Food Science Technology, Chinese Academy of Agricultural Sciences, Beijing 100094) Abstract: 【Objective】 To the changing law of stable carbon isotope composition in cattle tail hair with the change of feeds composition, and to verify each section of cattle tail hair can’t exchange with other sections once it is produced, and it contains isotopic information which can reflect the cattle feeds information from the time when it is produced.【Method】The experiment of cattle model w designed by changing the feeds composition, the changed trends of δ13C values in different segment of cattle tail hair with the change of feeds composition were analyzed, and the relation of stable carbon isotope composition between cattle tail hair, feeds and individual were analyzed. 【Result】There was a very significant difference between stable carbon isotope composition in different cattle tail hair segments with the feeds composition changed, and the feeds had more effects on carbon isotopic composition of cattle tail hair than individual. The significant correlations were showed between δ13C values of cattle tail hair segment and feeds, and they were significantly correlated with the proportion of C4 plant of fe