single-step production of a recyclable nanobiocatalyst for organophosphate pesticides biodegradation using functionalized bacterial magnetosomes单步执行生产的可回收nanobiocatalyst有机磷农药生物降解利用功能化细菌的发展前景.pdfVIP
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single-step production of a recyclable nanobiocatalyst for organophosphate pesticides biodegradation using functionalized bacterial magnetosomes单步执行生产的可回收nanobiocatalyst有机磷农药生物降解利用功能化细菌的发展前景
Single-Step Production of a Recyclable Nanobiocatalyst
for Organophosphate Pesticides Biodegradation Using
Functionalized Bacterial Magnetosomes
1,3,4 2,3,4 ´ 1,3,4 1,3,4
Nicolas Ginet *, Romain Pardoux , Geraldine Adryanczyk , Daniel Garcia , Catherine
Brutesco1,3,4, David Pignol1,3,4
´ ´ ´ ´
1 CEA, DSV, IBEB, Laboratoire de Bioenergetique Cellulaire, Saint-Paul-lez-Durance, France, 2 CEA, DSV, IBEB, Laboratoire des Interactions Proteines Metal, Saint-Paul-lez-
´ ´ ´
Durance, France, 3 CNRS, UMR 6191 Biologie Vegetale Microbiologie Environnementale, Saint-Paul-lez-Durance, France, 4 Aix-Marseille Universite, Saint-Paul-lez-
Durance, France
Abstract
Enzymes are versatile catalysts in laboratories and on an industrial scale; improving their immobilization would be beneficial
to broadening their applicability and ensuring their (re)use. Lipid-coated nano-magnets produced by magnetotactic
bacteria are suitable for a universally applicable single-step method of enzyme immobilization. By genetically
functionalizing the membrane surrounding these magnetite particles with a phosphohydrolase, we engineered an easy-
to-purify, robust and recyclable biocatalyst to degrade ethyl-paraoxon, a commonly used pesticide. For this, we genetically
fused the opd gene from Flavobacterium sp. ATCC 27551 encoding a paraoxonase to mamC, an abundant protein of the
magneto
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