specific oncogenic activity of the src-family tyrosine kinase c-yes in colon carcinoma cells特定致癌活性的src-family酪氨酸激酶c-yes结肠细胞癌.pdfVIP

Specific Oncogenic Activity of the Src-Family Tyrosine Kinase c-Yes in Colon Carcinoma Cells 1. ´ 1¤. 2. 1¤ Florence Sancier , Aurelie Dumont , Audrey Sirvent , Ludmilla Paquay de Plater , Thomas 1 ´ 1 1 1 ´ 1 ´ ´ 1 Edmonds , Geraldine David , Michel Jan , Catherine de Montrion , Francis Coge , Stephane Leonce , 1 1 1 1 2 Michael Burbridge , Alain Bruno , Jean A. Boutin , Brian Lockhart , Serge Roche *, Francisco Cruzalegui1* ´ ´ 1 Institut de Recherches Servier, Croissy-sur-Seine, France, 2 Equipe labellisee LA LIGUE 2009, Centre de Recherche de Biochimie Macromoleculaire, UMR5237 Centre ´ National de la Recherche Scientifique et Universite de Montpellier, Montpellier, France Abstract c-Yes, a member of the Src tyrosine kinase family, is found highly activated in colon carcinoma but its importance relative to c-Src has remained unclear. Here we show that, in HT29 colon carcinoma cells, silencing of c-Yes, but not of c-Src, selectively leads to an increase of cell clustering associated with a localisation of b-catenin at cell membranes and a reduction of expression of b-catenin target genes. c-Yes silencing induced an increase in apoptosis, inhibition of growth in soft-agar and in mouse xenografts, inhib