specificity of mimotope-induced anti-high molecular weight-melanoma associated antigen (hmw-maa) antibodies does not ensure biological activity特异性mimotope-induced卤分子weight-melanoma相关抗原(hmw-maa)抗体不能确保生物活性.pdfVIP

Specificity of Mimotope-Induced Anti-High Molecular Weight-Melanoma Associated Antigen (HMW-MAA) Antibodies Does Not Ensure Biological Activity 1. 1. 1 1 1 2 Julia Latzka , Sonja Gaier , Gerlinde Hofstetter , Nina Balazs , Ursula Smole , Soldano Ferrone , Otto 1 1 3 1 Scheiner , Heimo Breiteneder , Hubert Pehamberger , Stefan Wagner * 1 Department of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria, 2 Department of Surgery, of Immunology and of Pathology, University of Pittsburgh Cancer Institute, Pittsburgh, Pennsylvania, United States of America, 3 Division of General Dermatology, Department of Dermatology, Medical University of Vienna, Vienna, Austria Abstract Vaccines based on peptide mimics (mimotopes) of conformational tumor antigen epitopes have been investigated for a variety of human tumors including breast cancer, tumors expressing the carcinoembryonic antigen, B cell lymphoma, neuroblastoma, and melanoma. In our previous work, we designed a vaccine based on a mimotope of the high molecular weight-melanoma associated antigen (HMW-MAA) that elicited HMW-MAA-specific antibodies (Abs) with anti-tumor activity in vitro and in vivo. In this study, we aimed to identify mimotopes of additional distinct HMW-MAA epitopes, since they could be used to construct a polymimotope melanoma vaccine. For this purpose, random peptide phage libraries were screened with the anti-HMW-MAA monoclonal antibodies (mAbs) VT80.12 and VF1-TP43 yielding one peptide ligand for each mAb. Both peptides inhibited the binding of the correspondin