standardization of the filovirus plaque assay for use in preclinical studies标准化的filovirus空斑实验在临床前研究中使用.pdfVIP

Viruses 2012, 4, 3511-3530; doi:10.3390/v4123511 OPEN ACCESS viruses ISSN 1999-4915 /journal/viruses Article Standardization of the Filovirus Plaque Assay for Use in Preclinical Studies Amy C. Shurtleff 1,†, Julia E. Biggins 2,†, Ashley E. Keeney 2,†, Elizabeth E. Zumbrun 3,†, 3 2 2 4 Holly A. Bloomfield , Ana Kuehne , Jennifer L. Audet , Kendra J. Alfson , Anthony Griffiths 4,†, Gene G. Olinger 2 and Sina Bavari 1,*, for the Filovirus Animal Nonclinical Group (FANG) Assay Working Group † 1 Integrated Toxicology Division, United States Army Medical Research Institute of Infectious Diseases, 1425 Porter Street, Frederick, MD 21702, USA; E-Mail: amy.c.shurtleff.ctr@ 2 Virology Division, United States Army Medical Research Institute of Infectious Diseases, 1425 Porter Street, Frederick, MD 21702, USA; E-Mails: julia.biggins@ (J.E.B.); ashley.keeney@ (A.E.K.); ana.kuehne@ (A.K.); jennifer.l.audet@ (J.L.A.); gene.olinger@ (G.G.O.) 3 Center for Aerobiological Studies, United States Army Medical Research Institute of Infectious Diseases, 1425 Porter Street, Frederick, MD 21702, USA; E-Mails: elizabeth.zumbrun@ (E.E.Z.); holly.a.bloomfield@ (H.A.B.) 4 Department of Virology and Immunology, Texas Biomedical Research Institute, San Antonio, TX 78227, USA; E-Mails: kalfson@ (K.J.A.); agriffiths@ (A.G.) † The FANG Assay Working Group is an interagency group is composed of the following institutes as well as those referenced with the authors above: Susan Garges and Aparna Kolhe