原创力文档sterol carrier protein-x gene and effects of sterol carrier protein-2 inhibitors on lipid uptake in manduca sexta甾醇载体蛋白质x基因的影响甾醇载波protein-2抑制剂在manduca sexta脂质吸收.pdfVIP
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sterol carrier protein-x gene and effects of sterol carrier protein-2 inhibitors on lipid uptake in manduca sexta甾醇载体蛋白质x基因的影响甾醇载波protein-2抑制剂在manduca sexta脂质吸收
Kim and Lan BMC Physiology 2010, 10:9
/1472-6793/10/9
R E S E A R C H A R T I C L E Open Access
Research article
Sterol carrier protein-x gene and effects of sterol
carrier protein-2 inhibitors on lipid uptake in
Manduca sexta
1 2
Min-Sik Kim and Que Lan*
Abstract
Background: Cholesterol uptake and transportation during the feeding larval stages are critical processes in insects
because they are auxotrophic for exogenous (dietary) cholesterol. The midgut is the main site for cholesterol uptake in
many insects. However, the molecular mechanism by which dietary cholesterol is digested and absorbed within the
midgut and then released into the hemolymph for transportation to utilization or storage sites is poorly understood.
Sterol carrier proteins (SCP), non-specific lipid transfer proteins, have been spec ulated to be involved in intracellular
cholesterol transfer and metabolism in vertebrates. Based on the high degree of homology in the conserved sterol
transfer domain to rat and human SCP-2, it is supposed that insect SCP-2 has a parallel function to vertebrate SCP-2.
Results: We identified the Manduca sexta sterol carrier protein-x and the sterol carrier protein-2 (MsSCP-x/SCP-2) gene
from the larval fat body and the midgut cDNAs. The MsSCP-x/SCP-2 protein has a high degree of homology in the SCP-
2 domain to other insects SCP-2. Transcripts of MsSCP-2 were detected at high levels in the midgut and the fat body of
M. sexta during the larval stages. Recombinant MsSCP-2 bound to NBD-cholesterol with high affinity, which was
suppressed by sterol carrier protein-2 inhibitors.
Conclusions: The results suggest that MsSCP-2 may function as a lipid carrier protein in vivo, and targeting insect SCP-
2 may be a viable approach for the development of new insecticides.
Background
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