structural-functional characterization and physiological significance of ferredoxin-nadp+ reductase from xanthomonas axonopodis pv. citristructural-functional ferredoxin-nadp +还原酶的特性和生理意义、黄axonopodis pv。.pdfVIP
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structural-functionalcharacterizationandphysiologicalsignificanceofferredoxin-nadpreductasefromxanthomonasaxonopodispv.citristructural-functionalferredoxin-nadp还原酶的特性和生理意义、黄axonopodispv。
Structural-Functional Characterization and Physiological
Significance of Ferredoxin-NADP+ Reductase from
Xanthomonas axonopodis pv. citri
´ . ´ . ´
Marıa Laura Tondo , Matıas A. Musumeci , Marıa Laura Delprato, Eduardo A. Ceccarelli*, Elena G.
Orellano*
´ ´ ´
Molecular Biology Division, Instituto de Biologıa Molecular y Celular de Rosario (IBR), CONICET, Facultad de Ciencias Bioquımicas y Farmaceuticas, Universidad Nacional de
Rosario, Rosario, Argentina
Abstract
Xanthomonas axonopodis pv. citri is a phytopathogen bacterium that causes severe citrus canker disease. Similar to other
phytopathogens, after infection by this bacterium, plants trigger a defense mechanism that produces reactive oxygen
species. Ferredoxin-NADP+ reductases (FNRs) are redox flavoenzymes that participate in several metabolic functions,
including the response to reactive oxygen species. Xanthomonas axonopodis pv. citri has a gene (fpr) that encodes for a FNR
(Xac-FNR) that belongs to the subclass I bacterial FNRs. The aim of this work was to search for the physiological role of this
enzyme and to characterize its structural and functional properties. The functionality of Xac-FNR was tested by cross-
complementation of a FNR knockout Escherichia coli strain, which exhibit high susceptibility to agents that produce an
abnormal accumulation of NO2-. Xac-FNR was able to substitute for the FNR in E. coli in its antioxidant role. The expression of
fpr in X. axonopodis pv. citri was assessed using semiquantitative RT-PCR and W
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