study of probe-sample distance for biomedical spectra measurement生物医学光谱probe-sample距离测量的研究.pdfVIP

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study of probe-sample distance for biomedical spectra measurement生物医学光谱probe-sample距离测量的研究.pdf

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study of probe-sample distance for biomedical spectra measurement生物医学光谱probe-sample距离测量的研究

Wang et al. BioMedical Engineering OnLine 2011, 10:95 /content/10/1/95 RESEARCH Open Access Study of probe-sample distance for biomedical spectra measurement 1* 2 2 2 Bowen Wang , Shuzhen Fan , Lei Li and Cong Wang * Correspondence: jnwbw@163. Abstract com 1Hospital of Shandong University, Background: Fiber-based optical spectroscopy has been widely used for biomedical No. 91, Shanda Bei Road, Jinan, 250100, P. R. China applications. However, the effect of probe-sample distance on the collection Full list of author information is efficiency has not been well investigated. available at the end of the article Method: In this paper, we presented a theoretical model to maximize the illumination and collection efficiency in designing fiber optic probes for biomedical spectra measurement. This model was in general applicable to probes with single or multiple fibers at an arbitrary incident angle. In order to demonstrate the theory, a fluorescence spectrometer was used to measure the fluorescence of human finger skin at various probe-sample distances. The fluorescence spectrum and the total fluorescence intensity were recorded. Results: The theoretical results show that for single fiber probes, contact measurement always provides the best results. While for multi-fiber probes, there is an optimal probe distance. When a 400- μm excitation fiber is used to delive