suppression of cancer progression by mgat1 shrna knockdown抑制癌症恶化的mgat1 shrna击倒.pdfVIP

Suppression of Cancer Progression by MGAT1 shRNA Knockdown 1 1 1 1 1 Reza Beheshti Zavareh , Mahadeo A. Sukhai , Rose Hurren , Marcela Gronda , Xiaoming Wang , 2 1 2 3 2 3 Craig D. Simpson , Neil Maclean , Francis Zih , Troy Ketela , Carol J. Swallow , Jason Moffat , 4 5 1 2 David R. Rose , Harry Schachter , Aaron D. Schimmer *, James W. Dennis * 1 Ontario Cancer Institute, Princess Margaret Hospital, and Department of Medical Biophysics, University of Toronto, Toronto, Ontario, Canada, 2 Samuel Lunenfeld Research Institute, Mt. Sinai Hospital, Molecular Genetics, University of Toronto, Toronto, Ontario, Canada, 3 Department of Molecular Genetics, Donnelly Centre for Cellular and Biomolecular Research, Toronto, Ontario, Canada, 4 Department of Biology, University of Waterloo, Waterloo, Ontario, Canada, 5 Program in Molecular Structure and Function, Hospital for Sick Children, University of Toronto, Toronto, Ontario, Canada Abstract Oncogenic signaling promotes tumor invasion and metastasis, in part, by increasing the expression of tri- and tetra- branched N-glycans. The branched N-glycans bind to galectins forming a multivalent lattice that enhances cell surface residency of growth factor receptors, and focal adhesion turnover. N-acetylglucosaminyltransferase I (MGAT1), the first branching enzyme in the pathway, is required for the addition of all subsequent branches. Here we have introduced MGAT1 shRNA into human HeLa cervical