surface-enhanced raman spectral measurements of 5-fluorouracil in saliva表面增强拉曼光谱测量5 -氟尿嘧啶的唾液.pdfVIP

Molecules 2008, 13, 2608-2627; DOI: 10.3390/molecule OPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Article Surface-enhanced Raman Spectral Measurements of 5-Fluorouracil in Saliva Stuart Farquharson 1,*, Alan Gift 1, Chetan Shende 1, Frank Inscore 1, Beth Ordway 2, 2 2 Carl Farquharson and John Murren 1 Real-Time Analyzers, Inc., Middletown, CT 06457, USA E-mails: Alan@ (A. G.), Chetan@ (C. S.), Inscore@ (F. I.) 2 Yale University, New Haven, CT 06520, USA * Author to whom correspondence should be addressed; E-mail: stu@; Tel. +1-860-635-9800; Fax: +1-860-635-9804. Received: 8 August 2008; in revised form: 16 October 2008 / Accepted: 20 October 2008 / Published: 22 October 2008 Abstract: The ability of surface-enhanced Raman spectroscopy (SERS) to measure 5- fluorouracil (5-FU) in saliva is presented. The approach is based on the capacity of Raman spectroscopy to provide a unique spectral signature for virtually every chemical, and the ability of SERS to provide μg/mL sensitivity. A simple sampling method, that employed 1- mm glass capillaries filled with silver-doped sol-gels, was developed to isolate 5-FU from potential interfering chemical components of saliva and simultaneously provide SERS- activity. The method involved treating a 1 mL saliva sample with 1 mL of acetic acid, drawing 10 μL of sample into a SERS-active capillary by syringe, and then measuring the SER spectrum. Qu