testing for differentially-expressed micrornas with errors-in-variables nonparametric regression测试差异表达的小分子核糖核酸与errors-in-variables非参数回归.pdfVIP

Testing for Differentially-Expressed MicroRNAs with Errors-in-Variables Nonparametric Regression 1 2 3 4 4 Bin Wang *, Shu-Guang Zhang , Xiao-Feng Wang , Ming Tan , Yaguang Xi * 1 Mathematics and Statistics Department, University of South Alabama, Mobile, Alabama, United States of America, 2 Department of Statistics and Finance, University of Science and Technology of China, Hefei, Anhui, People’s Republic of China, 3 Department of Quantitative Health Sciences, Cleveland Clinic Foundation, Cleveland, Ohio, United States of America, 4 Mitchell Cancer Institute, University of South Alabama, Mobile, Alabama, United States of America Abstract MicroRNA is a set of small RNA molecules mediating gene expression at post-transcriptional/translational levels. Most of well-established high throughput discovery platforms, such as microarray, real time quantitative PCR, and sequencing, have been adapted to study microRNA in various human diseases. The total number of microRNAs in humans is approximately 1,800, which challenges some analytical methodologies requiring a large number of entries. Unlike messenger RNA, the majority of microRNA ( w60%) maintains relatively low abundance in the cells. When analyzed using microarray, the signals of these low-expressed microRNAs are influenced by other non-specific signals including the background noise. It is crucial to distinguish the true microRNA signals from measurement errors in microRNA array data analysis. In this study, we propose a novel measurement error model-based normalization method and differentially-expressed microRNA detection method for microRNA profiling data acquired from locked nucleic acids (LNA) microRNA array. Compared with some existing methods, the proposed method significantly im