systematic comparison of constitutive promoters and the doxycycline-inducible promoter系统的比较本构推动者和doxycycline-inducible启动子.pdfVIP

Systematic Comparison of Constitutive Promoters and the Doxycycline-Inducible Promoter 1,2. 2. 2 2 3 1 Jane Yuxia Qin , Li Zhang , Kayla L. Clift , Imge Hulur , Andy Peng Xiang , Bing-Zhong Ren *, Bruce T. Lahn2* 1 School of Life Sciences, Northeast Normal University, Changchun, China, 2 Howard Hughes Medical Institute, Department of Human Genetics, University of Chicago, Chicago, Illinois, United States of America, 3 Center for Stem Cell Biology and Tissue Engineering, Sun Yat-sen University, The Key Laboratory for Stem Cells and Tissue Engineering, Ministry of Education, Guangzhou, China Abstract Constitutive promoters are used routinely to drive ectopic gene expression. Here, we carried out a systematic comparison of eight commonly used constitutive promoters (SV40, CMV, UBC, EF1A, PGK and CAGG for mammalian systems, and COPIA and ACT5C for Drosophila systems). We also included in the comparison the TRE promoter, which can be activated by the rtTA transcriptional activator in a doxycycline-inducible manner. To make our findings representative, we conducted the comparison in a variety of cell types derived from several species. We found that these promoters vary considerably from one another in their strength. Most promoters have fairly consistent strengths across different cell types, but the CMV promoter can vary considerably from cell type to cell type. At maximal induction, the TRE promoter is comparable to a strong constitutive promoter. These results should facilitate more rational choices of promoters in ectopic gene expression studies. Citation: Qin JY, Zhang L, Clift KL, Hulur I, Xiang AP, et al. (2010) Systematic Comparison of Constitutive Promoters and the Doxycycline-Inducible Promoter. PLoS ONE 5(5): e10611. doi:10.137