the bacterial nanorecorder engineering e. coli to function as a chemical recording device细菌nanorecorder工程大肠杆菌作为化学记录装置.pdfVIP

The Bacterial Nanorecorder: Engineering E. coli to Function as a Chemical Recording Device 1 1 1,2,3 Prasanna Bhomkar *, Wayne Materi , David S. Wishart 1 National Institute for Nanotechnology, Edmonton, Alberta, Canada, 2 Department of Computing Science, University of Alberta, Edmonton, Alberta, Canada, 3 Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada Abstract Synthetic biology is an emerging branch of molecular biology that uses synthetic genetic constructs to create man-made cells or organisms that are capable of performing novel and/or useful applications. Using a synthetic chemically sensitive genetic toggle switch to activate appropriate fluorescent protein indicators (GFP, RFP) and a cell division inhibitor (minC), we have created a novel E. coli strain that can be used as a highly specific, yet simple and inexpensive chemical recording device. This biological ‘‘nanorecorder’’ can be used to determine both the type and the time at which a brief chemical exposure event has occurred. In particular, we show that the short–term exposure (15–30 min) of cells harboring this synthetic genetic circuit to small molecule signals (anhydrotetracycline or IPTG) triggered long-term and uniform cell elongation, with cell length being directly proportional to the time elapsed following a brief chemical exposure. This work demonstrates that facile modification of an existing genetic toggle switch can be exploited to generate a robust, biologically-based ‘‘nanorecorder’’ that could potentially be adapted to detect, respond and record a wide range of chemical stimuli that may vary over time and space. Citation: Bhomkar P, Materi W, Wishart DS (2011) The Bacteria