the density of cd10 corresponds to commitment and progression in the human b lymphoid lineagecd10的密度对应的承诺和发展在人类b淋巴血统.pdfVIP

The Density of CD10 Corresponds to Commitment and Progression in the Human B Lymphoid Lineage 1 2 2 1 1 2 Michiko Ichii , Kenji Oritani , Takafumi Yokota , Qingzhao Zhang , Karla P. Garrett , Yuzuru Kanakura , Paul W. Kincade1* 1 Immunobiology and Cancer Program, Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma, United States of America, 2 Department of Hematology and Oncology, Osaka University Graduate School of Medicine, Osaka, Japan Abstract Background: Requirements for human B lymphopoiesis are still poorly understood, and that has hampered investigation of differentiation events. For example, there are few cell surface antigens that can be used as milestones of lineage progression. The CD10 ectoenzyme is one such marker and has been used to define CLP, but we found substantial tissue specific variations in CD10 levels, and there was no information about how that corresponded to differentiation options. Methodology/Principal Findings: The aim of the present study was to use recently developed culture methods to assess the nature and differentiation potential of progenitors sorted according to CD10 density from umbilical cord blood (CB), adult bone marrow (BM) or G-CSF mobilized peripheral blood (PB). Many CD34+ cells in BM express high levels of CD10, while low or low/negative CD10 densities were found on CD34+ cells in CB or G-CSF mobilized PB, respectively. The relative abundance of CD10Lo versus CD10Hi cells only accounts for some CB versus BM differences. Almost all of the CD34+ CD10Hi cells expressed CD19 and lymphocyte transcription factors and corresponded to loss of myeloid potential. A high degree of immunoglobulin DH-JH gene rearrangements was characteristic only of the CD10Hi su